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Differential expression of NMDA and AMPA receptor subunits in DARPP-32-containing neurons of the cerebral cortex, hippocampus and neostriatum of rats
- Source :
- Brain research. 998(2)
- Publication Year :
- 2004
-
Abstract
- Dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein, 32 kDa (DARPP-32) is a key element of dopamine/D1/DARPP-32/protein phosphatase-1 (PP-1) signaling cascades of mammalian brain. We are interested in the expression patterns of N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors in DARPP-32-containing neurons, which may constitute morphological basis for interaction between dopamine and ionotropic glutamate receptors in dopaminoceptive cells. Double immunofluorescence was performed to visualize neurons showing coexpression of DARPP-32 with NMDA or AMPA receptor subunits (i.e., NR1, NR2a/b, glutamate receptor subunit 1 [GluR1], GluR2/3, and GluR4) in the forebrains of rats. Distribution of DARPP-32-positive neurons completely or partially overlapped with that of NMDA receptor- or AMPA receptor-immunoreactive ones in the frontal and parietal cortex, hippocampus and neostriatum, and neurons double-labeled with DARPP-32/NR1, DARPP-32/NR2a/b, DARPP-32/GluR1, DARPP-32/GluR2/3, or DARPP-32/GluR4 immunoreactivity were numerously observed. Semiquantification analysis indicated that most of DARPP-32-containing neurons (86-98%) expressed NR1, NR2a/b and GluR2/3, while less of them (14-90%) expressed GluR1 and GluR4. Although high rates (90-98%) of DARPP-32-positive cells expressed NMDA receptors in all regions above, variant percentages of them expressing AMPA receptor subunits were observed among the cortex (54-90%), hippocampus (59-97%) and neostriatum (14-97%). The study presents differential expression patterns of NMDA and AMPA receptors in DARPP-32-postive neurons in these forebrain regions. Taken together with previous reports, the present data suggest that interaction between dopamine and glutamate receptors may occur in the dopaminoceptive neurons with distinct receptor compositions and may be involved in modulating neuronal properties and excitotoxicity in mammalian forebrain.
- Subjects :
- Male
medicine.medical_specialty
Dopamine and cAMP-Regulated Phosphoprotein 32
Excitotoxicity
Hippocampus
Fluorescent Antibody Technique
Kainate receptor
Nerve Tissue Proteins
AMPA receptor
Biology
medicine.disease_cause
Receptors, N-Methyl-D-Aspartate
Rats, Sprague-Dawley
Internal medicine
medicine
Animals
Receptors, AMPA
Receptor
Long-term depression
Molecular Biology
Cerebral Cortex
Neurons
General Neuroscience
Glutamate receptor
Brain
Phosphoproteins
Cell biology
Rats
Neostriatum
Endocrinology
nervous system
NMDA receptor
Neurology (clinical)
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 998
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Brain research
- Accession number :
- edsair.doi.dedup.....b1d09a646bf798978387b61ae26107ad