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Glutathione-S-transferase and p53 polymorphisms in cervical carcinogenesis

Authors :
Sadamu Noda
Junko Saito
Minoru Ueki
Yao Ching Hung
Osamu Nunobiki
Yoshito Terai
Masatsugu Ueda
Source :
Gynecologic Oncology. 96:736-740
Publication Year :
2005
Publisher :
Elsevier BV, 2005.

Abstract

Objective. To investigate the clinical significance of glutathione-S-transferase GSTM1, GSTT1 and p53 codon 72 polymorphisms in cervical carcinogenesis. Methods. GSTM1, GSTT1 and p53 codon 72 polymorphisms together with human papillomavirus (HPV) types were examined in a total of 198 cervical smear samples using multiplex polymerase chain reaction (PCR) and PCR restriction fragment length polymorphism (RFLP) techniques. Results. Forty-two patients with high-grade squamous intraepithelial lesion (HSIL) had higher frequency of high-risk HPV and null GSTT1 genotype than 102 with low-grade SIL (LSIL) and 54 controls. Thirty-one patients with HSIL had also statistically higher frequency of null GSTT1 genotype than 28 with LSIL among 69 patients with high-risk HPV. There was no statistical difference in p53 Arg, Arg/Pro and Pro genotypes between SILs and controls with or without high-risk HPV. Conclusion. GSTT1 null genotype in cervical cell samples may be associated with more severe precancerous lesions of the cervix in a Japanese population. The p53 codon 72 polymorphism is unlikely to be related to HPV status and the onset of cervical cancer.

Details

ISSN :
00908258
Volume :
96
Database :
OpenAIRE
Journal :
Gynecologic Oncology
Accession number :
edsair.doi.dedup.....b1c3f78e6a3f0aa3e045cee59bab5960