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Glutathione-S-transferase and p53 polymorphisms in cervical carcinogenesis
- Source :
- Gynecologic Oncology. 96:736-740
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- Objective. To investigate the clinical significance of glutathione-S-transferase GSTM1, GSTT1 and p53 codon 72 polymorphisms in cervical carcinogenesis. Methods. GSTM1, GSTT1 and p53 codon 72 polymorphisms together with human papillomavirus (HPV) types were examined in a total of 198 cervical smear samples using multiplex polymerase chain reaction (PCR) and PCR restriction fragment length polymorphism (RFLP) techniques. Results. Forty-two patients with high-grade squamous intraepithelial lesion (HSIL) had higher frequency of high-risk HPV and null GSTT1 genotype than 102 with low-grade SIL (LSIL) and 54 controls. Thirty-one patients with HSIL had also statistically higher frequency of null GSTT1 genotype than 28 with LSIL among 69 patients with high-risk HPV. There was no statistical difference in p53 Arg, Arg/Pro and Pro genotypes between SILs and controls with or without high-risk HPV. Conclusion. GSTT1 null genotype in cervical cell samples may be associated with more severe precancerous lesions of the cervix in a Japanese population. The p53 codon 72 polymorphism is unlikely to be related to HPV status and the onset of cervical cancer.
- Subjects :
- medicine.medical_specialty
Genotype
Uterine Cervical Neoplasms
Polymerase Chain Reaction
Gastroenterology
Internal medicine
Multiplex polymerase chain reaction
medicine
Humans
Genetic Predisposition to Disease
Clinical significance
Codon
Papillomaviridae
Cervix
Glutathione Transferase
Cervical cancer
Polymorphism, Genetic
biology
business.industry
Papillomavirus Infections
Obstetrics and Gynecology
Genes, p53
Uterine Cervical Dysplasia
medicine.disease
Molecular biology
female genital diseases and pregnancy complications
Tumor Virus Infections
Squamous intraepithelial lesion
Cell Transformation, Neoplastic
Glutathione S-transferase
medicine.anatomical_structure
Oncology
biology.protein
Female
Restriction fragment length polymorphism
business
Polymorphism, Restriction Fragment Length
Subjects
Details
- ISSN :
- 00908258
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- Gynecologic Oncology
- Accession number :
- edsair.doi.dedup.....b1c3f78e6a3f0aa3e045cee59bab5960