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The effect of physiological concentrations of sex hormones, insulin, and glucagon on growth of breast and prostate cells supplemented with unmodified human serum
- Source :
- In vitro cellulardevelopmental biology. Animal. 46(10)
- Publication Year :
- 2010
-
Abstract
- The majority of cell culture studies have assessed the effect of hormones on cancer cell growth using media supplemented with charcoal-treated fetal bovine serum (CTS). We aimed to determine whether using a system more reflective of the human condition by changing the charcoal-treated serum to an untreated pooled human serum (PHS) resulted in the same hormone responses in breast and prostate cell lines. MCF-7 breast cancer, MCF-10A non-transformed breast, and LNCaP prostate cancer cell lines supplemented with PHS were treated with high and low physiological concentrations of six hormones (17β-estradiol, dehydroepiandosterone (DHEA), dihydrotestosterone (DHT), testosterone, insulin, and glucagon). Cell growth was measured after 72 h of incubation. All hormones stimulated growth of MCF-7 cells (p
- Subjects :
- Male
Serum
medicine.medical_specialty
medicine.medical_treatment
Cell Culture Techniques
Breast Neoplasms
Biology
Glucagon
Internal medicine
Cell Line, Tumor
LNCaP
medicine
Humans
Insulin
skin and connective tissue diseases
Gonadal Steroid Hormones
Testosterone
Cell Proliferation
Analysis of Variance
Cell growth
Prostatic Neoplasms
Cell Biology
General Medicine
Culture Media
Endocrinology
Dihydrotestosterone
Female
hormones, hormone substitutes, and hormone antagonists
Fetal bovine serum
Developmental Biology
Hormone
medicine.drug
Subjects
Details
- ISSN :
- 1543706X
- Volume :
- 46
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- In vitro cellulardevelopmental biology. Animal
- Accession number :
- edsair.doi.dedup.....b1c02dd8c3682fa71eb10f4106608d87