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Neonatal Bisphenol A Exposure Affects the IgM Humoral Immune Response to 4T1 Breast Carcinoma Cells in Mice
- Source :
- International Journal of Environmental Research and Public Health, Volume 16, Issue 10, International Journal of Environmental Research and Public Health, Vol 16, Iss 10, p 1784 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- Bisphenol A (BPA) is an endocrine disruptor of estrogenic nature. During the early stages of development, any exposure to BPA can have long-term effects. In this work, we study the potential alterations to the humoral antitumor immune (IgM) response in adult life after a single neonatal exposure to BPA. Female syngeneic BALB/c mice were exposed to a single dose of BPA of 250 &mu<br />g/kg. Once sexual maturity was reached, a breast tumor was induced. After 25 days, the serum was obtained, and the populations of B cells in the spleen and lymph nodes were analyzed by flow cytometry. The reactivity of IgM was evaluated by 2D immunoblots. No significant changes were found in the B cell populations in the peripheral lymph nodes and the spleen. The level of ER&alpha<br />expression was not significantly different. However, the IgM reactivity was affected. In individuals treated with BPA, a decrease in the number of IgMs that recognize tumor antigens was observed. The possibility that these antibodies are the high affinity products of the adaptive response is discussed. The recognition of IgG was also evaluated but a null recognition was found in the controls as in the individuals treated with the 4T1 cells.
- Subjects :
- endocrine system
IgM
Health, Toxicology and Mutagenesis
lcsh:Medicine
Spleen
Endocrine Disruptors
Article
Flow cytometry
Andrology
03 medical and health sciences
Mice
0302 clinical medicine
Immune system
4T1 cells
Antigen
Phenols
Cell Line, Tumor
medicine
Animals
Benzhydryl Compounds
B cell
030304 developmental biology
0303 health sciences
Mice, Inbred BALB C
biology
medicine.diagnostic_test
urogenital system
lcsh:R
Public Health, Environmental and Occupational Health
Estrogen Receptor alpha
Mammary Neoplasms, Experimental
Environmental Exposure
BPA
Immunity, Humoral
medicine.anatomical_structure
Endocrine disruptor
Immunoglobulin M
030220 oncology & carcinogenesis
biology.protein
Female
Lymph
Antibody
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- Language :
- English
- ISSN :
- 16604601 and 16617827
- Volume :
- 16
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- International Journal of Environmental Research and Public Health
- Accession number :
- edsair.doi.dedup.....b1a7a652ed6c519cf8d9a5a0388dd43f