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RNA interference shows that Spook, the precursor gene of 20-hydroxyecdysone (20E), regulates the molting of Macrobrachium nipponense
- Source :
- The Journal of Steroid Biochemistry and Molecular Biology. 213:105976
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The aim of this study was to explore the function of the Mn-Spook gene, which was found in the ovary transcriptome of the Oriental river prawn (Macrobrachium nipponense). The Spook gene, which is the precursor gene of 20-hydroxyecdysone (20E), plays an important role in the process of molting in many arthropods, but its function in M. nipponense is unclear. We cloned the full-length Mn-Spook gene from the ovary of M. nipponense and found that it had the same conserved domains as the P450 gene of the Halloween family of genes. The Mn-Spook gene was highly expressed in ovary and gill tissue during the breeding period. During ovarian development, Mn-spook gene expression was highest at the nearly-ripe stage, and it also was highly expressed in the zoea developmental stage. Cellular localization analysis showed that Mn-Spook signals accumulated in the cytoplasmic membrane and nucleus of oocytes. Finally, we used RNA interference to evaluate the function of the Mn-Spook gene. Compared with the control group, in vivo injection of Mn-Spook dsRNA effectively downregulated the expression of Mn-Spook and the content of 20E. The molting frequency of M. nipponense in the experimental group also was significantly inhibited. These results demonstrated that the Mn-Spook gene played an important role in the molting process of M. nipponense.
- Subjects :
- Endocrinology, Diabetes and Metabolism
Genetic Vectors
Clinical Biochemistry
20-Hydroxyecdysone
Gene Expression
Molting
Biology
Biochemistry
Arthropod Proteins
Transcriptome
chemistry.chemical_compound
Endocrinology
Cytochrome P-450 Enzyme System
RNA interference
Gene expression
Animals
Amino Acid Sequence
Cloning, Molecular
Molecular Biology
Gene
Phylogeny
Cellular localization
Sequence Homology, Amino Acid
Ovary
Gene Expression Regulation, Developmental
Cell Biology
Recombinant Proteins
Cell biology
RNA silencing
Ecdysterone
chemistry
Oocytes
Molecular Medicine
Female
RNA Interference
Palaemonidae
Macrobrachium nipponense
Sequence Alignment
Subjects
Details
- ISSN :
- 09600760
- Volume :
- 213
- Database :
- OpenAIRE
- Journal :
- The Journal of Steroid Biochemistry and Molecular Biology
- Accession number :
- edsair.doi.dedup.....b1a06f130089f4d87c4c7c5e0c7d0eff