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Electroacupuncture ameliorates poloxamer 407-induced hyperlipidemia through suppressing hepatic SREBP-2 expression in rats
- Source :
- Life Sciences. 203:20-26
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Aims Acupuncture, particularly electroacupuncture (EA) has been shown to have the lipid-lowering effects, but not completely investigated. The present study was aimed to examine whether EA could attenuate poloxamer-407 (P-407)-induced hyperlipidemia in the rats and to investigate its potential mechanisms. Main methods Rats received P-407 (0.4 g/kg, i.p.) to induce hyperlipidemia. EA was performed at ST36 and ST40 acupoints a total of three times with 12 h-interval starting 1 h before the P-407 injection at 0.6 mA intensity and 2 Hz frequency for 10 min. Key findings In P-407-induced hyperlipidemic rats, EA stimulation at ST36 and ST40 acupoints significantly lowered the serum levels of triglycerides, total cholesterol, LDL-cholesterol and atherogenic index, while markedly increasing the serum HDL-cholesterol levels. Meanwhile, hyperlipidemic rats had significantly higher expression of sterol regulatory element-binding protein (SREBP)-2, without any difference in SREBP-1 expression in the liver, as compared with normal ones. EA significantly attenuated the expression of SREBP-2 with a subsequent decrease in 3-hydroxy-3-methylglutaryl coenzyme A reductase and an increase in low-density lipoprotein receptor at both mRNA and protein levels in the liver of hyperlipidemic rats. These changes did not occur after electrical stimulation at a non-acupoint. Significance Taken together, our findings indicate that EA stimulation to P-407-induced hyperlipidemic rats improves the lipid abnormalities, which may be associated with regulation of the expression of key enzymes of cholesterol synthesis in the liver through modulation of SREBP-2.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Electroacupuncture
medicine.medical_treatment
Hyperlipidemias
Stimulation
Poloxamer
030204 cardiovascular system & hematology
Reductase
General Biochemistry, Genetics and Molecular Biology
Rats, Sprague-Dawley
Surface-Active Agents
03 medical and health sciences
0302 clinical medicine
Internal medicine
Hyperlipidemia
medicine
Animals
General Pharmacology, Toxicology and Pharmaceutics
Receptor
Chemistry
General Medicine
medicine.disease
Lipids
Sterol
Rats
Sterol regulatory element-binding protein
030104 developmental biology
Endocrinology
Gene Expression Regulation
lipids (amino acids, peptides, and proteins)
Sterol Regulatory Element Binding Protein 2
Lipoprotein
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 203
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....b19f91407ba4db20def83af9b331c811