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Age-related alteration of gap junction distribution and connexin expression in rat aortic endothelium
- Source :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 48(10)
- Publication Year :
- 2000
-
Abstract
- We investigated endothelial gap junctions and their three component connexins, connexin37 (Cx37), Cx40, and Cx43, during growth and senescence in rat aorta by en face immunoconfocal microscopy and electron microscopy. Gap junction spots labeled by specific antisera against Cx37, Cx40, and Cx43 were quantified at 1 day, 7 days, 28 days, 16 months, and ≥20 months of age, and the relationship between the connexins was examined by co-localization analysis. At birth, all three connexins were abundantly expressed; the number and total area of connexin spots then declined within 1 week ( p < 0.05 for each connexin). From 1 week, each connexin showed a distinct temporal expression pattern. Whereas Cx43 signal decreased progressively, Cx37 signal fluctuated in a downward trend. By contrast, Cx40 maintained an abundant level until ≥20 months of age (≥20 months vs 28 days, p < 0.05 for number and total connexin signal area). These patterns were associated with changes in endothelial cell morphology. Double-label analysis showed that the extent of co-localization of connexins to the same gap junctional spot was age-dependent [>70% at birth and 28 days old
- Subjects :
- 0301 basic medicine
Senescence
Aging
Histology
Blotting, Western
Connexin
Fluorescent Antibody Technique
Biology
Connexins
Andrology
Rats, Sprague-Dawley
03 medical and health sciences
medicine.artery
medicine
Distribution (pharmacology)
Animals
Aorta
Microscopy, Confocal
030102 biochemistry & molecular biology
Immune Sera
Gap junction
Gap Junctions
Cell biology
Rats
Endothelial stem cell
Microscopy, Electron
030104 developmental biology
Animals, Newborn
Aortic endothelium
sense organs
Endothelium, Vascular
Anatomy
Intracellular
Subjects
Details
- ISSN :
- 00221554
- Volume :
- 48
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
- Accession number :
- edsair.doi.dedup.....b193fa92dd9a64c1d0ec44de50a2536b