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Direct Detection of Viable but Non-culturable (VBNC) Salmonella in Real Food System by a Rapid and Accurate PMA-CPA Technique
- Source :
- Frontiers in Microbiology, Vol 12 (2021), Frontiers in Microbiology
- Publication Year :
- 2021
- Publisher :
- Frontiers Media S.A., 2021.
-
Abstract
- Salmonella enterica is a typical foodborne pathogen with multiple toxic effects, including invasiveness, endotoxins, and enterotoxins. Viable but nonculturable (VBNC) is a type of dormant form preserving the vitality of microorganisms, but it cannot be cultured by traditional laboratory techniques. The aim of this study is to develop a propidium monoazide-crossing priming amplification (PMA-CPA) method that can successfully detect S. enterica rapidly with high sensitivity and can identify VBNC cells in food samples. Five primers (4s, 5a, 2a/1s, 2a, and 3a) were specially designed for recognizing the specific invA gene. The specificity of the CPA assay was tested by 20 different bacterial strains, including 2 standard S. enterica and 18 non-S. enterica bacteria strains covering Gram-negative and Gram-positive isolates. Except for the two standard S. enterica ATCC14028 and ATCC29629, all strains showed negative results. Moreover, PMA-CPA can detect the VBNC cells both in pure culture and three types of food samples with significant color change. In conclusion, the PMA-CPA assay was successfully applied on detecting S. enterica in VBNC state from food samples.
- Subjects :
- Microbiology (medical)
Salmonella
Microorganism
lcsh:QR1-502
Enterotoxin
medicine.disease_cause
Microbiology
Viable but nonculturable
lcsh:Microbiology
03 medical and health sciences
viable but non-culturable (VBNC)
medicine
propidium monoazide (PMA)
Original Research
030304 developmental biology
0303 health sciences
biology
Foodborne pathogen
030306 microbiology
Salmonella enterica
biology.organism_classification
rapid detection
crossing priming amplification (CPA)
Pure culture
Bacteria
Subjects
Details
- Language :
- English
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology
- Accession number :
- edsair.doi.dedup.....b193bff8e937f9dc025663aae45c726b