Back to Search
Start Over
Prevalence and Phylogenetic Analysis ofOrientia tsutsugamushiin Small Mammals in Hanoi, Vietnam
- Source :
- Vector-Borne and Zoonotic Diseases. 16:96-102
- Publication Year :
- 2016
- Publisher :
- Mary Ann Liebert Inc, 2016.
-
Abstract
- Rodents are important reservoirs of many human pathogens transmitted via arthropod vectors. Arthropod-borne bacteria belonging to the family Rickettsiaceae cause acute febrile diseases in humans worldwide, but the real burdens of rickettsial diseases appear to be underestimated in Hanoi, Vietnam, because differential diagnosis on the basis of clinical signs and symptoms is confounded by the presence of other tropical infectious diseases with similar signs and symptoms. To know the prevalence of bacteria of the family Rickettsiaceae among small mammals in Hanoi, 519 animals thriving in the public places were captured and examined for the presence of bacterial sequences using duplex PCR. Nucleotide sequences specific for Orientia tsutsugamushi were detected in seven samples (1.3%). Out of seven animals, two were captured in a market, whereas five were in hospitals. None of the captured small mammals tested positive for the genus Rickettsia. The nucleotide sequence analysis of the genes encoding the 47-kDa high-temperature requirement A (47-kDa HtrA) and 56-kDa type-specific antigen (TSA) showed that these seven isolates were indistinguishable from each other. O. tsutsugamushi isolated in this study was closely related phylogenetically to the Gilliam strain, which was originally isolated at the border of Assam and Burma, rather than to those isolated in the central to southern part of Vietnam. It should be emphasized that Vietnamese hospitals were heavily infested by small rodents and some of them harbored O. tsutsugamushi. Strict hygienic control should be implemented to mitigate the potential risk posed by O. tsutsugamushi in hospital settings.
- Subjects :
- 0301 basic medicine
Orientia tsutsugamushi
030231 tropical medicine
Rodentia
Human pathogen
Scrub typhus
Disease Vectors
Biology
Microbiology
03 medical and health sciences
Hospitals, Urban
0302 clinical medicine
Phylogenetics
Virology
Prevalence
medicine
Animals
Phylogeny
Phylogenetic tree
Shrews
Zoonosis
Nucleic acid sequence
bacterial infections and mycoses
medicine.disease
biology.organism_classification
030104 developmental biology
Infectious Diseases
Scrub Typhus
Vietnam
Arthropod Vector
Subjects
Details
- ISSN :
- 15577759 and 15303667
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Vector-Borne and Zoonotic Diseases
- Accession number :
- edsair.doi.dedup.....b181ad5a4b593d659720f07fe7078968