Back to Search
Start Over
Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1
- Source :
- Carbohydrate Research. 408:18-24
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids. LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp. longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively. LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, was shown to release GNB from Gb5 and GA1 oligosaccharides. This is the first report describing enzymatic release of β-linked GNB from natural substrates. These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
- Subjects :
- Substrate Specificities
Bifidobacterium longum
Glycoside Hydrolases
Stereochemistry
ved/biology.organism_classification_rank.species
Oligosaccharides
Biochemistry
Substrate Specificity
Analytical Chemistry
Bacterial Proteins
Antigens, Tumor-Associated, Carbohydrate
Glycoside hydrolase
Trisaccharide
Sugar
chemistry.chemical_classification
Bifidobacterium bifidum
biology
Chemistry
ved/biology
Organic Chemistry
General Medicine
biology.organism_classification
Enzyme
Bifidobacterium
Galacto-N-biose
Subjects
Details
- ISSN :
- 00086215
- Volume :
- 408
- Database :
- OpenAIRE
- Journal :
- Carbohydrate Research
- Accession number :
- edsair.doi.dedup.....b17f00b0fcdfc2fd77e02136cbe8e1b6