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Impact of Tilapia hepcidin 2-3 dietary supplementation on the gut microbiota profile and immunomodulation in the grouper (Epinephelus lanceolatus)
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-17 (2019), Scientific Reports
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Hepcidin regulates iron homeostasis and host-defense mechanisms, while the hepcidin-like protein, Tilapia hepcidin (TH)2-3, functions as an antimicrobial peptide (AMP). Since AMP dietary supplements may be used as alternatives to antibiotics in livestock, we tested the effects of recombinant (r)TH2-3 as a dietary supplement in grouper aquaculture. rTH2-3 was produced by a Pichia pastoris expression system and exhibited thermostability and broad-spectrum antimicrobial activity. The feed conversion ratio and feed efficiency were determined in Epinephelus lanceolatus (grouper) fed with rTH2-3-supplemented diet for 28 days. In addition, grouper showed enhanced superoxide dismutase (SOD) activity after rTH2-3 feeding compared to regular-diet-fed fish. Gut microbiota analysis revealed that microbial diversity was enhanced by feeding grouper with 1% rTH2-3. After challenging grouper with Vibrio alginolyticus, differential regulation of immune-related genes in the liver and spleen was observed between the TH2-3 and regular-diet groups, including for genes associated with antimicrobial and pro-inflammatory functions, complement components, and major histocompatibility complex (Mhc). These findings suggest that overall immunity was improved. Thus, our results suggest long-term supplementation with rTH2-3 may be beneficial for aquacultured grouper. The beneficial effects of the supplement are likely based on changes in the commensal microbial community as well as immunomodulation.
- Subjects :
- 0301 basic medicine
food.ingredient
Animal feed
lcsh:Medicine
Microbial Sensitivity Tests
Gut flora
Antimicrobial resistance
Feed conversion ratio
Article
Microbiology
Immunomodulation
03 medical and health sciences
food
Anti-Infective Agents
Hepcidins
Hepcidin
Animals
Grouper
lcsh:Science
Vibrio alginolyticus
Multidisciplinary
Bacteria
biology
Protein Stability
lcsh:R
Temperature
Tilapia
Feeding Behavior
04 agricultural and veterinary sciences
Epinephelus
biology.organism_classification
Animal Feed
Recombinant Proteins
Gastrointestinal Microbiome
030104 developmental biology
Gene Expression Regulation
Dietary Supplements
Fermentation
040102 fisheries
biology.protein
0401 agriculture, forestry, and fisheries
Bass
lcsh:Q
Metagenomics
Infection
Spleen
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....b1604ac701705794f1df3ad1b6f5e9ff