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Interactions between the microbiome and mating influence the female’s transcriptional profile in Drosophila melanogaster
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Drosophila melanogaster females undergo a variety of post-mating changes that influence their activity, feeding behavior, metabolism, egg production and gene expression. These changes are induced either by mating itself or by sperm or seminal fluid proteins. In addition, studies have shown that axenic females—those lacking a microbiome—have altered fecundity compared to females with a microbiome, and that the microbiome of the female’s mate can influence reproductive success. However, the extent to which post-mating changes in transcript abundance are affected by microbiome state is not well-characterized. Here we investigated fecundity and the post-mating transcript abundance profile of axenic or control females after mating with either axenic or control males. We observed interactions between the female’s microbiome and her mating status: transcripts of genes involved in reproduction and genes with neuronal functions were differentially abundant depending on the females’ microbiome status, but only in mated females. In addition, immunity genes showed varied responses to either the microbiome, mating, or a combination of those two factors. We further observed that the male’s microbiome status influences the fecundity of both control and axenic females, while only influencing the transcriptional profile of axenic females. Our results indicate that the microbiome plays a vital role in the post-mating switch of the female’s transcriptome.
- Subjects :
- Male
0301 basic medicine
030106 microbiology
Reproductive biology
lcsh:Medicine
Microbiology
Article
Transcriptome
Sexual Behavior, Animal
03 medical and health sciences
Animals
Germ-Free Life
RNA-Seq
Microbiome
Mating
Transcriptomics
lcsh:Science
Axenic
reproductive and urinary physiology
Genetics
Multidisciplinary
Host Microbial Interactions
biology
Reproductive success
Axenic Culture
Microbiota
Reproduction
lcsh:R
biology.organism_classification
Fecundity
Sperm
Immunity, Innate
Drosophila melanogaster
Fertility
030104 developmental biology
behavior and behavior mechanisms
Female
lcsh:Q
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....b15eba46e232862ed293b643316fdb3e