Moving acid boundary (pH gradient) paper ionophoresis of bile salts

METHODS have been devised for the separation of bile acids and their conjugates with glycine or taurine using partition chromatography on columns1,2 or paper3,4. These methods, however, may confuse certain glycine conjugated acids with the unconjugated bile acids containing a greater number of hydroxyl groups4. Thus ambiguous separations may result if unconjugated bile acids are also present in the mixture.