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Using Raman spectroscopy to characterize biological materials
- Source :
- Nature Protocols. 11:664-687
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Raman spectroscopy can be used to measure the chemical composition of a sample, which can in turn be used to extract biological information. Many materials have characteristic Raman spectra, which means that Raman spectroscopy has proven to be an effective analytical approach in geology, semiconductor, materials and polymer science fields. The application of Raman spectroscopy and microscopy within biology is rapidly increasing because it can provide chemical and compositional information, but it does not typically suffer from interference from water molecules. Analysis does not conventionally require extensive sample preparation; biochemical and structural information can usually be obtained without labeling. In this protocol, we aim to standardize and bring together multiple experimental approaches from key leaders in the field for obtaining Raman spectra using a microspectrometer. As examples of the range of biological samples that can be analyzed, we provide instructions for acquiring Raman spectra, maps and images for fresh plant tissue, formalin-fixed and fresh frozen mammalian tissue, fixed cells and biofluids. We explore a robust approach for sample preparation, instrumentation, acquisition parameters and data processing. By using this approach, we expect that a typical Raman experiment can be performed by a nonspecialist user to generate high-quality data for biological materials analysis.
- Subjects :
- Sample (material)
Biocompatible Materials
02 engineering and technology
Spectrum Analysis, Raman
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
Specimen Handling
symbols.namesake
Interference (communication)
Microscopy
Animals
Sample preparation
Instrumentation (computer programming)
Mammals
Electronic Data Processing
Data processing
Data Collection
010401 analytical chemistry
Plants
021001 nanoscience & nanotechnology
Biological materials
0104 chemical sciences
Biochemistry
symbols
0210 nano-technology
Biological system
Raman spectroscopy
Subjects
Details
- ISSN :
- 17502799 and 17542189
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Nature Protocols
- Accession number :
- edsair.doi.dedup.....b10802e516b2b4b6c550aa26ae7a43de