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Microglia: An Intrinsic Component of the Proliferative Zones in the Fetal Rhesus Monkey (Macaca mulatta) Cerebral Cortex
- Source :
- Barger, N; Keiter, J; Kreutz, A; Krishnamurthy, A; Weidenthaler, C; Martínez-Cerdeño, V; et al.(2018). Microglia: An Intrinsic Component of the Proliferative Zones in the Fetal Rhesus Monkey (Macaca mulatta) Cerebral Cortex.. Cerebral cortex (New York, N.Y. : 1991). doi: 10.1093/cercor/bhy145. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/7193b05v, Cereb Cortex, Cerebral cortex (New York, N.Y. : 1991), vol 29, iss 7
- Publication Year :
- 2018
- Publisher :
- eScholarship, University of California, 2018.
-
Abstract
- Microglial cells are increasingly recognized as modulators of brain development. We previously showed that microglia colonize the cortical proliferative zones in the prenatal brain and regulate the number of precursor cells through phagocytosis. To better define cellular interactions between microglia and proliferative cells, we performed lentiviral vector-mediated intraventricular gene transfer to induce enhanced green fluorescent protein expression in fetal cerebrocortical cells. Tissues were collected and counterstained with cell-specific markers to label microglial cells and identify other cortical cell types. We found that microglial cells intimately interact with the radial glial scaffold and make extensive contacts with neural precursor cells throughout the proliferative zones, particularly in the rhesus monkey fetus when compared to rodents. We also identify a subtype of microglia, which we term ‘periventricular microglia’, that interact closely with mitotic precursor cells in the ventricular zone. Our data suggest that microglia are structural modulators that facilitate remodeling of the proliferative zones as precursor cells migrate away from the ventricle and may facilitate the delamination of precursor cells. Taken together, these results indicate that microglial cells are an integral component of cortical proliferative zones and contribute to the interactive milieu in which cortical precursor cells function.
- Subjects :
- Brain development
Neurogenesis
Cognitive Neuroscience
Phagocytosis
radial glial cells
rhesus monkey
Biology
050105 experimental psychology
03 medical and health sciences
Cellular and Molecular Neuroscience
Fetus
0302 clinical medicine
microglial cells
Neural Stem Cells
Precursor cell
medicine
Psychology
Animals
cortical development
proliferative zones
0501 psychology and cognitive sciences
Mitosis
Cerebral Cortex
Microglia
05 social sciences
Neurosciences
Experimental Psychology
Macaca mulatta
Rats
Cell biology
medicine.anatomical_structure
Cerebral cortex
Cognitive Sciences
Original Article
030217 neurology & neurosurgery
Function (biology)
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Barger, N; Keiter, J; Kreutz, A; Krishnamurthy, A; Weidenthaler, C; Martínez-Cerdeño, V; et al.(2018). Microglia: An Intrinsic Component of the Proliferative Zones in the Fetal Rhesus Monkey (Macaca mulatta) Cerebral Cortex.. Cerebral cortex (New York, N.Y. : 1991). doi: 10.1093/cercor/bhy145. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/7193b05v, Cereb Cortex, Cerebral cortex (New York, N.Y. : 1991), vol 29, iss 7
- Accession number :
- edsair.doi.dedup.....b0f9bc1d2209acd2886bd3d35afebfc9