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Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization
- Source :
- Data in Brief, Data in Brief, Vol 7, Iss, Pp 60-65 (2016)
- Publication Year :
- 2016
- Publisher :
- Elsevier, 2016.
-
Abstract
- Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.
- Subjects :
- 0301 basic medicine
viruses
030106 microbiology
Immunocytochemistry
lcsh:Computer applications to medicine. Medical informatics
Virus
law.invention
03 medical and health sciences
law
2.2 Factors relating to the physical environment
Aetiology
lcsh:Science (General)
Data Article
chemistry.chemical_classification
Multidisciplinary
biology
Virology
030104 developmental biology
Enzyme
Infectious Diseases
Capsid
chemistry
Polyclonal antibodies
Recombinant DNA
biology.protein
Immunohistochemistry
lcsh:R858-859.7
Antibody
lcsh:Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 23523409
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Data in Brief
- Accession number :
- edsair.doi.dedup.....b0f833db10158417c73552f08e896113