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Molecular and functional properties of lung SP cells
- Source :
- American Journal of Physiology-Lung Cellular and Molecular Physiology. 292:L972-L983
- Publication Year :
- 2007
- Publisher :
- American Physiological Society, 2007.
-
Abstract
- Previous analysis of lung injury and repair has provided evidence for region-specific stem cells that maintain proximal and distal epithelial compartments. However, redundant expression of lineage markers by cells at several levels of the stem cell hierarchy has complicated phenotypic and functional characterization of clonogenic airway cells. Based on the demonstration that rapid efflux of the DNA dye Hoechst 33342 can be used to prospectively purify long-term repopulating hematopoietic stem cells, we hypothesized that lung cells with similar biochemical properties would be enriched for clonogenic progenitors. We demonstrate that Hoechst-dim side population (SP) cells isolated from proximal and distal compartments of the mouse lung were relatively small and agranular, exhibited low red and green autofluorescence, and that the SP fraction was highly enriched in clonogenic cells. Quantitative RT-PCR indicated that vimentin mRNA was enriched and that epithelial markers were depleted in these preparations of SP cells. Bleomycin exposure was associated with decreased clonogenicity among alveolar SP and suggested that SP cell function was compromised under profibrotic conditions. We conclude that the SP phenotype is common to clonogenic cells at multiple airway locations and suggest that Hoechst efflux is a property of cells expressing a wound-repair phenotype.
- Subjects :
- Male
Pulmonary and Respiratory Medicine
Pathology
medicine.medical_specialty
Physiology
Vimentin
Lung injury
Mice
Side population
Physiology (medical)
medicine
Animals
Uteroglobin
Progenitor cell
Clonogenic assay
Lung
Staining and Labeling
biology
Stem Cells
Lineage markers
Keratin-14
Epithelial Cells
Cell Biology
Molecular biology
Clone Cells
Pulmonary Alveoli
Trachea
Haematopoiesis
Phenotype
biology.protein
Benzimidazoles
Female
Stem cell
Subjects
Details
- ISSN :
- 15221504 and 10400605
- Volume :
- 292
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Lung Cellular and Molecular Physiology
- Accession number :
- edsair.doi.dedup.....b0e38d825e4a79c802e2d1371960e0f6