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Effects and mode of action of chitosan and ivy fruit saponins on the microbiome, fermentation and methanogenesis in the rumen simulation technique
- Source :
- FEMS Microbiology Ecology
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- This study investigates the effects of supplementing a control diet (CON) with chitosan (CHI) or ivy fruit saponins (IVY) as natural feed additives. Both additives had similar abilities to decrease rumen methanogenesis (–42% and –40%, respectively) using different mechanisms: due to its antimicrobial and nutritional properties CHI promoted a shift in the fermentation pattern towards propionate production which explained about two thirds of the decrease in methanogenesis. This shift was achieved by a simplification of the structure in the bacterial community and a substitution of fibrolytic (Firmicutes and Fibrobacteres) by amylolytic bacteria (Bacteroidetes and Proteobacteria) which led to greater amylase activity, lactate and microbial protein yield with no detrimental effect on feed digestibility. Contrarily, IVY had negligible nutritional properties promoting minor changes in the fermentation pattern and on the bacterial community. Instead, IVY modified the structure of the methanogen community and decreased its diversity. This specific antimicrobial effect of IVY against methanogens was considered its main antimethanogenic mechanism. IVY had however a negative impact on microbial protein synthesis. Therefore, CHI and IVY should be further investigated in vivo to determine the optimum doses which maintain low methanogenesis but prevent negative effects on the rumen fermentation and animal metabolism.<br />Rumen function is generally suboptimal leading to loses in the form of methane and nitrogen, analysis of the rumen microbiome is vital to understand the mode of action of new feed additives to improve rumen function.
- Subjects :
- feed additive
Chemoautotrophic Growth
Rumen
Methanogenesis
Firmicutes
Feed additive
Euryarchaeota
Applied Microbiology and Biotechnology
Microbiology
ivy fruit saponins
RNA, Ribosomal, 16S
Botany
Animals
Food science
Amylase
chemistry.chemical_classification
Chitosan
Bacteria
Base Sequence
Ecology
biology
Microbiota
methane
High-Throughput Nucleotide Sequencing
Sequence Analysis, DNA
Saponins
biology.organism_classification
Methanogen
Anti-Bacterial Agents
rumen fermentation
chemistry
Fruit
Dietary Supplements
Fermentation
Propionate
biology.protein
Propionates
Research Article
Subjects
Details
- ISSN :
- 15746941
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Ecology
- Accession number :
- edsair.doi.dedup.....b0d2d0fa6f82c6ca239f13e1408c718c