Back to Search
Start Over
Salidroside protects against ventilation-induced lung injury by inhibiting the expression of matrix metalloproteinase-9
- Source :
- Pharmaceutical Biology, Vol 59, Iss 1, Pp 760-768 (2021), Pharmaceutical Biology, article-version (VoR) Version of Record
- Publication Year :
- 2021
- Publisher :
- Taylor & Francis Group, 2021.
-
Abstract
- Context Salidroside, a compound extracted from Rhodiola rosea L. (Crassulaceae), possesses many beneficial pathological effects. Objective To explore the effect of salidroside on ventilator-induced lung endothelial dysfunction in vivo and in vitro. Materials and methods In vivo, male ICR mice were divided into sham, ventilation, salidroside, and ventilation plus salidroside groups. The mice were ventilated for 4 h, salidroside (50 mg/kg) was administrated intraperitoneally before ventilation, dexamethasone (Dex) (5 mg/kg) was used as a positive control. In vitro, mouse lung vascular endothelial cells (MLVECs) were treated with salidroside, MMP-9 siRNA, and BAY11-7082 (10 μM), and then exposed to cyclic stretch for 4 h. Afterward, lung tissues and MLVECs were collected for further analysis. Results Salidroside pre-treatment significantly reversed the expression of vascular endothelial cadherin (VE-cadherin) and zonula occluden-1 (ZO-1) proteins in cyclic stretch-treated MLVECs (0.46 ± 0.09 vs. 0.80 ± 0.14, 0.49 ± 0.05 vs. 0.88 ± 0.08) and ventilated lung tissues (0.56 ± 0.06 vs. 0.83 ± 0.46, 0.49 ± 0.08 vs. 0.80 ± 0.12). The results further indicated that salidroside inhibited the expression of matrix metalloproteinase-9 (MMP-9), whereas knockdown of its expression restored the expression levels of VE-cadherin (0.37 ± 0.08 vs. 0.85 ± 0.74) and ZO-1 (0.48 ± 0.08 vs. 0.81 ± 0.11) in stretched MLVECs. Meanwhile, salidroside inhibited the NF-κB signalling pathway and alleviated lung injury. Conclusions Salidroside protected against stretch-induced endothelial barrier function, improving lung injury after ventilation. Thus, salidroside may be a promising therapeutic agent for patients with MV-induced lung injury.
- Subjects :
- Male
Ventilator-Induced Lung Injury
Pharmaceutical Science
Context (language use)
RM1-950
Lung injury
Pharmacology
Dexamethasone
endothelial dysfunction
nf-κb signalling pathway
Mice
chemistry.chemical_compound
Glucosides
Phenols
In vivo
Drug Discovery
medicine
Animals
Endothelial dysfunction
Mice, Inbred ICR
biology
Chemistry
Salidroside
NF-kappa B
Endothelial Cells
General Medicine
biology.organism_classification
medicine.disease
Rhodiola rosea
Matrix Metalloproteinase 9
Complementary and alternative medicine
acute lung injury
Gene Knockdown Techniques
Breathing
Molecular Medicine
Rhodiola
Therapeutics. Pharmacology
Signal Transduction
Research Article
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 17445116 and 13880209
- Volume :
- 59
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical Biology
- Accession number :
- edsair.doi.dedup.....b0d020249edb9307fa060123c5d196de