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Suppressed immune microenvironment and repertoire in brain metastases from patients with resected non-small-cell lung cancer
- Source :
- Ann Oncol
- Publication Year :
- 2019
- Publisher :
- Oxford University Press, 2019.
-
Abstract
- BACKGROUND: The tumor immune microenvironment (TIME) of lung cancer brain metastasis is largely unexplored. We carried out immune profiling and sequencing analysis of paired resected primary tumors and brain metastases of non-small-cell lung carcinoma (NSCLC). PATIENTS AND METHODS: TIME profiling of archival formalin-fixed and paraffin-embedded specimens of paired primary tumors and brain metastases from 39 patients with surgically resected NSCLCs was carried out using a 770 immune gene expression panel and by T-cell receptor beta repertoire (TCRβ) sequencing. Immunohistochemistry was carried out for validation. Targeted sequencing was carried out to catalog hot spot mutations in cancer genes. RESULTS: Somatic hot spot mutations were mostly shared between both tumor sites (28/39 patients; 71%). We identified 161 differentially expressed genes, indicating inhibition of dendritic cell maturation, Th1, and leukocyte extravasation signaling pathways, in brain metastases compared with primary tumors (P
- Subjects :
- 0301 basic medicine
Adult
Male
Receptors, Antigen, T-Cell, alpha-beta
Tumor-associated macrophage
03 medical and health sciences
0302 clinical medicine
Immune system
Carcinoma, Non-Small-Cell Lung
medicine
Carcinoma
Biomarkers, Tumor
Tumor Microenvironment
Humans
Lung cancer
Aged
Tumor microenvironment
business.industry
Brain Neoplasms
Hematology
Dendritic cell
Original Articles
Dendritic Cells
Middle Aged
medicine.disease
Immunohistochemistry
Neoplasm Proteins
Gene Expression Regulation, Neoplastic
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Mutation
Cancer research
Female
business
Brain metastasis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Ann Oncol
- Accession number :
- edsair.doi.dedup.....b0c4d924bad8ac65a413e4793ad73903