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Crohn's disease patients have more IgG-binding fecal bacteria than controls
- Source :
- Clinical and vaccine immunology, 19(4), 515-521
- Publication Year :
- 2012
-
Abstract
- In Crohn's disease (CD), chronic gut inflammation leads to loss of mucosal barrier integrity. Subsequent leakage of IgG to the gut could produce an increase of IgG coating of intestinal bacteria. We investigated if there is more IgG coating in patients than in volunteers and whether this is dependent on the host IgG response or on the gut bacteria. Fecal and serum samples were obtained from 23 CD patients and 11 healthy volunteers. Both thein vivoIgG-coated fecal bacteria andin vitroIgG coating after serum addition were measured by flow cytometry and related to disease activity. The bacterial composition in feces was determined using fluorescencein situhybridization. The IgG-binding capacities ofEscherichia colistrains isolated from feces of patients and volunteers were assessed. The results showed that thein vivoIgG-coated fraction of fecal bacteria of patients was slightly larger than that of volunteers but significantly larger after incubation with either autologous or heterologous serum. This was dependent on the bacteria and independent of disease activity or the serum used. The presence of moreEnterobacteriaceaeand fewer faecalibacteria in patient feces was confirmed.E. coliisolates from patients bound more IgG than isolates from volunteers (P< 0.05) after the addition of autologous serum. Together, these results indicate that CD patients have more IgG-binding gut bacteria than healthy volunteers. We showed that the level of IgG coating depends on the bacteria and not on the serum used. Furthermore, CD patients have a strong specific immune response to their ownE. colibacteria.
- Subjects :
- Microbiology (medical)
MUCOSA-ASSOCIATED MICROBIOTA
Adult
Male
Clinical Biochemistry
Immunology
Biology
medicine.disease_cause
Severity of Illness Index
Immunoglobulin G
Microbiology
Feces
Young Adult
Crohn Disease
Enterobacteriaceae
medicine
Immunology and Allergy
Humans
Escherichia coli
In Situ Hybridization, Fluorescence
Aged
Human feces
TARGETED OLIGONUCLEOTIDE PROBES
RNA-BASED PROBES
IN-SITU HYBRIDIZATION
Middle Aged
biology.organism_classification
Flow Cytometry
CALPROTECTIN
Antibodies, Bacterial
ULCERATIVE-COLITIS
IgG binding
ESCHERICHIA-COLI
biology.protein
POPULATIONS
Female
Clinical Immunology
Antibody
Bacteria
HUMAN FECES
INFLAMMATORY-BOWEL-DISEASE
Subjects
Details
- ISSN :
- 1556679X and 15566811
- Volume :
- 19
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Clinical and vaccine immunology : CVI
- Accession number :
- edsair.doi.dedup.....b0aa48b15fa932e043f5b9100496cc47