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Validation of a novel approach for the rapid production of immunogenic virus-like particles for bluetongue virus
- Source :
- Vaccine, Vaccine, Elsevier, 2010, 28 (17), pp.3047-3054. ⟨10.1016/j.vaccine.2009.10.072⟩
- Publication Year :
- 2010
- Publisher :
- HAL CCSD, 2010.
-
Abstract
- International audience; Bluetongue virus causes an emerging disease of ruminants, principally affecting sheep and cattle. Since 1998, there have been multiple separate outbreaks of bluetongue disease in Europe that have highlighted the need for a safe, efficacious, DIVA compliant vaccine. We report here a new baculovirus expression strategy which allowed pre-integration of the genes encoding the BTV inner capsid proteins at one baculovirus locus and those encoding the outer capsid proteins at a different locus. A modified baculovirus with two marker proteins to facilitate the phenotypic selection of recombinant viruses was developed. The utility of this approach is demonstrated by the production of BTV VLPs to a number of serotypes. For a proof of concept, VLPs of one serotype was then tested for protective immune response. VLPs were demonstrated to be safe, highly effective, immunogens in sheep, reducing post-challenge viraemia to levels below the threshold detection limit of quantitative RT-PCR when vaccinated animals were challenged with virulent virus.
- Subjects :
- Male
Serotype
Virosomes
viruses
[SDV]Life Sciences [q-bio]
Genetic Vectors
Gene Expression
Virulence
Reoviridae
Virus
Cell Line
Viral Proteins
03 medical and health sciences
Virus-like particle
Bluetongue disease
VIRUS-LIKE PARTICLES
Cricetinae
medicine
Animals
Viremia
Cloning, Molecular
030304 developmental biology
0303 health sciences
Sheep
Orbivirus
General Veterinary
General Immunology and Microbiology
biology
030306 microbiology
Immunogenicity
Public Health, Environmental and Occupational Health
Viral Vaccines
BLUETONGUE VIRUS
biology.organism_classification
medicine.disease
Virology
3. Good health
Vaccines, Virosome
Infectious Diseases
Molecular Medicine
BACULOVIRUS EXPRESSION
Female
Baculoviridae
Subjects
Details
- Language :
- English
- ISSN :
- 0264410X
- Database :
- OpenAIRE
- Journal :
- Vaccine, Vaccine, Elsevier, 2010, 28 (17), pp.3047-3054. ⟨10.1016/j.vaccine.2009.10.072⟩
- Accession number :
- edsair.doi.dedup.....b0a68b6277b0a5b1ec5bc491cde333d3