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Interferon-γ-mediated secretion of tryptophanyl-tRNA synthetases has a role in protection of human umbilical cord blood-derived mesenchymal stem cells against experimental colitis
- Source :
- BMB Reports
- Publication Year :
- 2019
- Publisher :
- Korean Society for Biochemistry and Molecular Biology, 2019.
-
Abstract
- Mesenchymal stem cells (MSCs) are multipotent adult stem cells that present immunosuppressive effects in experimental and clinical trials targeting various rare diseases including inflammatory bowel disease (IBD). In addition, recent studies have reported tryptophanyl-tRNA synthetase (WRS) possesses uncanonical roles such as angiostatic and anti-inflammatory effects. However, little is known about the function of WRS in MSC-based therapy. In this study, we investigated if a novel factor, WRS, secreted from MSCs has a role in amelioration of IBD symptoms and determined a specific mechanism underlying MSC therapy. Experimental colitis was induced by administration of 3% DSS solution to 8-week-old mice and human umbilical cord blood-derived MSCs (hUCB-MSCs) were injected intraperitoneally. Secretion of WRS from hUCB-MSCs and direct effect of WRS on isolated CD4+ T cells was determined via in vitro experiments and hUCB-MSCs showed significant therapeutic rescue against experimental colitis. Importantly, WRS level in serum of colitis induced mice decreased and recovered by administration of MSCs. Through in vitro examination, WRS expression of hUCB-MSCs increased when cells were treated with interferon-γ (IFN-γ). WRS was evaluated and revealed to have a role in inhibiting activated T cells by inducing apoptosis. In summary, IFN-γ- mediated secretion of WRS from MSCs has a role in suppressive effect on excessive inflammation and disease progression of IBD and brings new highlights in the immunomodulatory potency of hUCB-MSCs. [BMB Reports 2019; 52(5): 318-323]
- Subjects :
- CD4-Positive T-Lymphocytes
Male
medicine.medical_treatment
Inflammation
Tryptophan-tRNA Ligase
Mesenchymal Stem Cell Transplantation
Biochemistry
Inflammatory bowel disease
Interferon-gamma
Mice
medicine
Animals
Humans
Colitis
Molecular Biology
Stem cell therapy
business.industry
Stem Cells
Mesenchymal stem cell
Mesenchymal Stem Cells
General Medicine
Stem-cell therapy
Articles
medicine.disease
Fetal Blood
In vitro
Mice, Inbred C57BL
Disease Models, Animal
Apoptosis
Cancer research
Aminoacyl-tRNA synthetase
Interferon-γ
medicine.symptom
business
Adult stem cell
Subjects
Details
- Language :
- English
- ISSN :
- 1976670X and 19766696
- Volume :
- 52
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....b0a53fbc199bd255ff4be73865819763