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Estrogen receptor-α polymorphism in a Taiwanese clinical breast cancer population: a case–control study
- Source :
- Breast Cancer Research
- Publication Year :
- 2004
- Publisher :
- BioMed Central, 2004.
-
Abstract
- Introduction Receptor-mediated estrogen activation participates in the development and progression of breast cancer. Estrogen receptor (ER)-α polymorphism has been found to be associated with breast cancer and clinical features of the disease in Caucasians. Epidemiologic studies have revealed that age–incidence patterns of breast cancer in Asians differ from those in Caucasians. Genomic data for ER-α in either population is therefore of value in the clinical setting for that ethnic group. Methods A case–control study was conducted to establish a database of ER-α polymorphisms in a Taiwanese population in order to compare Western and Taiwanese (Asian) distributions and to evaluate ER-α polymorphism as an indicator of clinical outcome. The ER-α gene was scanned in a Taiwanese clinical breast cancer group (189 patients) and in healthy individuals (177 healthy control individuals). PCR single-strand conformation polymorphism technology was employed and real-time PCR melting curve analysis was performed. Results Three sites of silent single nucleotide polymorphism (SNPs) were found, as reported previously in Western studies, but at significantly different frequencies. Among the three SNPs, the frequency of allele 1 (TCT → TCC) in codon 10 was significantly lower in breast cancer patients (32.0%) than in control individuals (40.4%; P = 0.018). We found that allele 1 (ACG → ACA) in codon 594 was less common in breast cancer patients with a family history of breast cancer (5.9%) than in those without such a history (19.6%; P = 0.049). Individually, both allele 1 in codon 325 (CCC → CCG) and allele 1 in codon 594 exhibited a reverse association with the occurrence of lymph node metastasis. Furthermore, incorporation of both SNP markers further increased predictive accuracy. Conclusions Our data suggest that ER-α polymorphisms are correlated with various aspects of breast cancer in Taiwan. ER-α genotype, as determined during presurgical evaluation, might represent a surrogate marker for predicting breast cancer lymph node metastasis.
- Subjects :
- Oncology
Adult
medicine.medical_specialty
Genotype
medicine.drug_class
Population
Taiwan
Estrogen receptor
Breast Neoplasms
Disease
Biology
Polymorphism, Single Nucleotide
Metastasis
polymorphism
Breast cancer
Asian People
Gene Frequency
Surgical oncology
estrogen receptor-α
Internal medicine
medicine
metastasis
Humans
Genetic Predisposition to Disease
education
Codon
Alleles
Polymorphism, Single-Stranded Conformational
Aged
Medicine(all)
Aged, 80 and over
education.field_of_study
Reverse Transcriptase Polymerase Chain Reaction
Case-control study
Estrogen Receptor alpha
Exons
lymph node
Middle Aged
medicine.disease
Neoplasm Proteins
Treatment Outcome
Receptors, Estrogen
Estrogen
Case-Control Studies
Lymphatic Metastasis
Immunology
Female
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 1465542X and 14655411
- Volume :
- 6
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Breast Cancer Research
- Accession number :
- edsair.doi.dedup.....b08c1a1a1ee5d0b0219e01ebfe49cc01