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Effects of Superoxide Dismutase on the Acetylcholine-induced Relaxation Response in Cholesterol-fed and Streptozotocin-induced Diabetic Mice
- Source :
- Journal of Smooth Muscle Research. 35:33-46
- Publication Year :
- 1999
- Publisher :
- Japan Society of Smooth Muscle Research, 1999.
-
Abstract
- High concentration of acetylcholine (ACh) caused a rapid and long lasting relaxation response in age-matched controls, whereas this response was significantly weaker in streptozotocin (STZ)-diabetic and cholesterol fed mice. The levels of basal and ACh-stimulated cyclic GMP in the aorta was also significantly smaller in STZ-diabetic and cholesterol-fed mice. The attenuated relaxation responses to ACh in both STZ-diabetic and cholesterol-fed mice were ameliorated by the chronic administration of cholestyramine. A prior incubation of aortic strips with superoxide dismutase (SOD, 60 U/ml) improved the recovery phase of the relaxation of diabetic aorta after single administration of ACh, whereas SOD had no effects on ACh-induced relaxation of aortic strips from cholesterol fed mice. These results suggest that superoxide anion may be responsible for an impairment of endothelium-dependent relaxation of aorta from STZ induced diabetic mice. It is further suggested that impairment of endothelium-dependent relaxation in STZ-diabetic and cholesterol fed mice may be caused by different mechanisms.
- Subjects :
- Blood Glucose
Male
medicine.medical_specialty
endocrine system diseases
Endothelium
Physiology
Muscle Relaxation
Cholestyramine Resin
Radioimmunoassay
In Vitro Techniques
Nitric Oxide
Muscle, Smooth, Vascular
Streptozocin
Diabetes Mellitus, Experimental
Superoxide dismutase
Mice
chemistry.chemical_compound
Internal medicine
medicine.artery
medicine
Animals
Cyclic GMP
Aorta
Analysis of Variance
Mice, Inbred ICR
Cholestyramine
biology
Relaxation (psychology)
Superoxide Dismutase
Superoxide
Anticholesteremic Agents
General Medicine
Streptozotocin
Acetylcholine
Cholesterol
Endocrinology
medicine.anatomical_structure
chemistry
biology.protein
medicine.drug
Subjects
Details
- ISSN :
- 18848796 and 09168737
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Journal of Smooth Muscle Research
- Accession number :
- edsair.doi.dedup.....b07b36a7cb3bb31373613820b0f994d7
- Full Text :
- https://doi.org/10.1540/jsmr.35.33