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Effects of Superoxide Dismutase on the Acetylcholine-induced Relaxation Response in Cholesterol-fed and Streptozotocin-induced Diabetic Mice

Authors :
Masato Nakajima
Katsuo Kamata
Makoto Sugiura
Source :
Journal of Smooth Muscle Research. 35:33-46
Publication Year :
1999
Publisher :
Japan Society of Smooth Muscle Research, 1999.

Abstract

High concentration of acetylcholine (ACh) caused a rapid and long lasting relaxation response in age-matched controls, whereas this response was significantly weaker in streptozotocin (STZ)-diabetic and cholesterol fed mice. The levels of basal and ACh-stimulated cyclic GMP in the aorta was also significantly smaller in STZ-diabetic and cholesterol-fed mice. The attenuated relaxation responses to ACh in both STZ-diabetic and cholesterol-fed mice were ameliorated by the chronic administration of cholestyramine. A prior incubation of aortic strips with superoxide dismutase (SOD, 60 U/ml) improved the recovery phase of the relaxation of diabetic aorta after single administration of ACh, whereas SOD had no effects on ACh-induced relaxation of aortic strips from cholesterol fed mice. These results suggest that superoxide anion may be responsible for an impairment of endothelium-dependent relaxation of aorta from STZ induced diabetic mice. It is further suggested that impairment of endothelium-dependent relaxation in STZ-diabetic and cholesterol fed mice may be caused by different mechanisms.

Details

ISSN :
18848796 and 09168737
Volume :
35
Database :
OpenAIRE
Journal :
Journal of Smooth Muscle Research
Accession number :
edsair.doi.dedup.....b07b36a7cb3bb31373613820b0f994d7
Full Text :
https://doi.org/10.1540/jsmr.35.33