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The graft content of hyaluronan is increased during xenograft rejection
- Source :
- Xenotransplantation. 11:269-275
- Publication Year :
- 2004
- Publisher :
- Wiley, 2004.
-
Abstract
- One main hurdle to xenotransplantation, i.e. transplantation between different species, is the immunological barrier that the organ meets in the recipient. The aim of this thesis was to characterise xenogeneic rejection mechanisms by using the concordant mouse-to-rat heart transplantation model.Graft-infiltrating immune cells could be isolated from both rejecting and non-rejecting grafts using ex vivo propagation, a technique based on incubation of graft biopsies in culture medium for 48 hours. The numbers of recovered T lymphocytes were considerably higher in grafts undergoing cell-mediated rejection than in grafts undergoing acute vascular rejection (AVR) or in non-rejecting transplants. Thus, ex vivo propagation should be a valuable tool for further studies of cell-mediated rejection.Cytokine patterns in the grafts, as measured by a quantitative real-time RT-PCR method, showed that AVR and cell-mediated rejection are associated with an increase of both pro-inflammatory cytokines (IL-1β and TNF-α) and more specific cytokines (IL-2, IL-10, IL-12p40 and IFN-γ). These data differed considerably from the patterns seen in the spleens of the recipients. Cell-mediated xenograft rejection was also found to be associated with a local accumulation of hyaluronan.Oral administration of xenogeneic cells stimulated a production of antibodies that could induce hyperacute rejection of cardiac xenografts when passively transferred to graft recipients. This is in contrast to several models for autoimmune diseases and allogeneic transplantation where oral administration of antigens is an effective way to induce unresponsiveness. Hence, future attempts to induce oral tolerance in xenotransplantation should be done with caution.
- Subjects :
- Graft Rejection
Allogeneic transplantation
medicine.medical_treatment
Xenotransplantation
Transplantation, Heterologous
Immunology
Mice
Immune system
Antigen
Reference Values
medicine
Animals
Hyaluronic Acid
Heart transplantation
Transplantation
biology
business.industry
Myocardium
Rats
biology.protein
Heart Transplantation
Antibody
business
Immunosuppressive Agents
Ex vivo
Subjects
Details
- ISSN :
- 13993089 and 0908665X
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Xenotransplantation
- Accession number :
- edsair.doi.dedup.....b079b54f45e58ae1879f8f7d237b3b3e
- Full Text :
- https://doi.org/10.1111/j.1399-3089.2004.00132.x