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Enrichment of processed pseudogene transcripts in L1-ribonucleoprotein particles
- Source :
- Human Molecular Genetics. 22:3730-3748
- Publication Year :
- 2013
- Publisher :
- Oxford University Press (OUP), 2013.
-
Abstract
- Long INterspersed Elements (LINE-1s, L1s) are responsible for over one million retrotransposon insertions and 8000 processed pseudogenes (PPs) in the human genome. An active L1 encodes two proteins (ORF1p and ORF2p) that bind with L1 RNA and form L1-ribonucleoprotein particles (RNPs). Although it is believed that the RNA-binding property of ORF1p is critical to recruit other mobile RNAs to the RNP, the identity of recruited RNAs is largely unknown. Here, we used crosslinking and immunoprecipitation followed by deep sequencing to identify RNA components of L1-RNPs. Our results show that in addition to retrotransposed RNAs [L1, Alu and SINE-VNTR-Alu (SVA)], L1-RNPs are enriched with cellular mRNAs, which have PPs in the human genome. Using purified L1-RNPs, we show that PP-source RNAs preferentially serve as ORF2p templates in a reverse transcriptase assay. In addition, we find that exogenous ORF2p binds endogenous ORF1p, allowing reverse transcription of the same PP-source RNAs. These data demonstrate that interaction of a cellular RNA with the L1-RNP is an inside track to PP formation.
- Subjects :
- Retroelements
Pseudogene
Gene Expression
Retrotransposon
Biology
Open Reading Frames
Gene expression
Genetics
Humans
Small nucleolar RNA
Molecular Biology
Genetics (clinical)
Ribonucleoprotein
High-Throughput Nucleotide Sequencing
RNA-Binding Proteins
RNA
RNA-Directed DNA Polymerase
Articles
General Medicine
Molecular biology
Long non-coding RNA
Long interspersed nuclear element
HEK293 Cells
Long Interspersed Nucleotide Elements
Ribonucleoproteins
Pseudogenes
Subjects
Details
- ISSN :
- 14602083 and 09646906
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Human Molecular Genetics
- Accession number :
- edsair.doi.dedup.....b053851bb9981df57745b9ab184bb095