Back to Search
Start Over
TLR2-dependent amelioration of allergic airway inflammation by parasitic nematode type II MIF in mice
- Source :
- Parasite Immunology. 37:180-191
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- In our previous studies, the recombinant type II macrophage migration inhibitory factor homologue (rAs-MIF) secreted from Anisakis simplex suppressed experimental inflammation mouse model through IL-10 production and CD4(+)CD25(+)Foxp3(+) T-cell recruitment. Also, TLR2 gene expression was significantly increased following rAs-MIF treatment. To know the relation between TLR2 and amelioration mechanisms of rAs-MIF, we induced allergic airway inflammation by ovalbumin and alum with or without rAs-MIF under TLR2 blocking systems [anti-TLR2-specific antibody (α-mTLR2 Ab) treatment and using TLR2 knockout mice]. As a result, the amelioration effects of rAs-MIF in allergic airway inflammation model (diminished inflammation and Th2 response in the lung, increased IL-10 secretion, CD4(+)CD25(+)Foxp3(+) T-cell recruitment) were diminished under two of the TLR2 blocking model. The expression of TLR2 on the surface of lung epithelial cell was significantly elevated by rAs-MIF treatment or Pam3CSK (TLR2-specific agonist) treatment, but they might have some competition effect on the elevation of TLR2 expression. In addition, the elevation of IL-10 gene expression by rAs-MIF treatment was significantly inhibited by α-mTLR2 Ab or Pam3CSK pretreatment. In conclusion, anti-inflammatory effects of the rAs-MIF on OVA-induced allergic airway inflammation might be closely related to TLR2.
- Subjects :
- Agonist
Ovalbumin
medicine.drug_class
Immunology
chemical and pharmacologic phenomena
Inflammation
Biology
T-Lymphocytes, Regulatory
Mice
Gene expression
Hypersensitivity
otorhinolaryngologic diseases
medicine
Animals
Secretion
Lung
Macrophage Migration-Inhibitory Factors
Mice, Knockout
Helminth Proteins
T-Lymphocytes, Helper-Inducer
respiratory system
Anisakis
Toll-Like Receptor 2
Interleukin-10
Mice, Inbred C57BL
Disease Models, Animal
TLR2
Knockout mouse
biology.protein
Alum Compounds
Female
Parasitology
Macrophage migration inhibitory factor
medicine.symptom
Subjects
Details
- ISSN :
- 01419838
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Parasite Immunology
- Accession number :
- edsair.doi.dedup.....b03572394ffbea75182a7b6d6a52a097