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Detection, isolation and characterization of Fusobacterium gastrosuis sp. nov. colonizing the stomach of pigs
- Source :
- Systematic and Applied Microbiology. 40:42-50
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Nine strains of a novel Fusobacterium sp. were isolated from the stomach of 6-8 months old and adult pigs. The isolates were obligately anaerobic, although they endured 2h exposure to air. Phylogenetic analysis based on 16S rRNA and gyrase B genes demonstrated that the isolates showed high sequence similarity with Fusobacterium mortiferum, Fusobacterium ulcerans, Fusobacterium varium, Fusobacterium russii and Fusobacterium necrogenes, but formed a distinct lineage in the genus Fusobacterium. Comparative analysis of the genome of the type strain of this novel Fusobacterium sp. confirmed that it is different from other recognized Fusobacterium spp. DNA-DNA hybridization, fingerprinting and genomic %GC determination further supported the conclusion that the isolates belong to a new, distinct species. The isolates were also distinguishable from these and other Fusobacterium spp. by phenotypical characterization. The strains produced indole and exhibited proline arylamidase and glutamic acid decarboxylase activity. They did not hydrolyse esculin, did not exhibit pyroglutamic acid arylamidase, valine arylamidase, α-galactosidase, β-galactosidase, β-galactosidase-6-phosphate or α-glucosidase activity nor produced acid from cellobiose, glucose, lactose, mannitol, mannose, maltose, raffinose, saccharose, salicin or trehalose. The major fatty acids were C16:0 and C18:1ω9c. The name Fusobacterium gastrosuis sp. nov. is proposed for the novel isolates with the type strain CDW1(T) (=DSM 101753(T)=LMG 29236(T)). We also demonstrated that Clostridium rectum and mortiferum Fusobacterium represent the same species, with nomenclatural priority for the latter.
- Subjects :
- DNA, Bacterial
0301 basic medicine
Swine
Biology
DNA, Ribosomal
Applied Microbiology and Biotechnology
Microbiology
03 medical and health sciences
Cytosol
Clostridium
Fusobacterium mortiferum
stomatognathic system
Fusobacterium ulcerans
RNA, Ribosomal, 16S
Clostridium rectum
Animals
Cluster Analysis
Anaerobiosis
Phylogeny
Ecology, Evolution, Behavior and Systematics
Base Composition
Fatty Acids
Stomach
Nucleic Acid Hybridization
Sequence Analysis, DNA
Fusobacterium
16S ribosomal RNA
biology.organism_classification
Bacterial Typing Techniques
stomatognathic diseases
030104 developmental biology
Fusobacterium necrogenes
Fusobacterium Infections
Fusobacterium russii
Subjects
Details
- ISSN :
- 07232020
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Systematic and Applied Microbiology
- Accession number :
- edsair.doi.dedup.....b03030c26a455ae7e452c6d38f1decac