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Polyethylenimine-grafted polycarbonates as biodegradable polycations for gene delivery
- Source :
- Biomaterials. 30:4824-4832
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Polycations as one of non-viral vectors have gained increasing attentions. In this paper, polyethylenimine(PEI)-grafted polycarbonates (PMAC-g-PEIx) were synthesized as a kind of biodegradable polycations for gene delivery. Backbone polymer, poly(5-methyl-5-allyloxycarbonyl-trimethylene carbonate) (PMAC), was synthesized in bulk catalyzed by immobilized porcine pancreas lipase (IPPL). Then, PMAC-O, the allyl epoxidation product of PMAC, was further modified by PEIx with low molecular weight (x = 423, 800 and 1800). The MWs of PMAC-g-PEIx, measured by GPC-MALLS, were 81,900, 179,900 and 200,600 g/mol with polydispersities of 1.2, 1.4 and 1.7, respectively. PMAC-g-PEIx could form positively charged nano-sized particles (30-90 nm) with pDNA, and all the three PAMC-g-PEIx/DNA polyplexes had similar buffer capabilities. In vitro experiments demonstrated that the PAMC-g-PEIx showed much low cytotoxicity and enhanced transfection efficiency could be found in comparison with PEI25K in 293T cells. Furthermore, pre-incubation of PMAC-g-PEI1800 showed a weakening binding capacity with DNA. The biodegradability of PMAC-g-PEIx can facilitate the efficient release of pDNA from polyplexes and reduce cell cytotoxicity. These results suggested that PMAC-g-PEIx would be a promising non-viral biodegradable vector for gene delivery system.
- Subjects :
- Magnetic Resonance Spectroscopy
Materials science
Cell Survival
Green Fluorescent Proteins
Biophysics
Biocompatible Materials
Bioengineering
Buffers
Gene delivery
Transfection
Cell Line
Biomaterials
chemistry.chemical_compound
Microscopy, Electron, Transmission
Polymer chemistry
Polyamines
Humans
Polyethyleneimine
Lipase
Luciferases
Cytotoxicity
Electrophoresis, Agar Gel
chemistry.chemical_classification
Polyethylenimine
Polycarboxylate Cement
Cell Death
biology
Gene Transfer Techniques
DNA
Polymer
Biodegradation
Polyelectrolytes
Combinatorial chemistry
Polyelectrolyte
chemistry
Mechanics of Materials
Ceramics and Composites
biology.protein
Biological Assay
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....b0114391e0ac530cd7cd87b35cdfeb96