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Lactobacillus rhamnosus GG soluble mediators ameliorate early life stress-induced visceral hypersensitivity and changes in spinal cord gene expression
- Source :
- Neuronal Signaling
- Publication Year :
- 2020
- Publisher :
- Portland Press Ltd., 2020.
-
Abstract
- Visceral hypersensitivity is a hallmark of many functional and stress-related gastrointestinal disorders, and there is growing evidence that the gut microbiota may play a role in its pathophysiology. It has previously been shown that early life stress-induced visceral sensitivity is reduced by various probiotic strains of bacteria (including Lactobacillus rhamnosus GG (LGG)) alone or in combination with prebiotic fibres in rat models. However, the exact mechanisms underpinning such effects remain unresolved. Here, we investigated if soluble mediators derived from LGG can mimic the bacteria’s effects on visceral hypersensitivity and the microbiota–gut–brain axis. Rats were exposed to maternal separation (MS) from postnatal days 2–12. From weaning onwards both non-separated (NS) and MS offspring were provided drinking water with or without supplementation of standardized preparations of the LGG soluble mediators (LSM). Our results show that MS led to increased visceral sensitivity and exaggerated corticosterone plasma levels following restraint stress in adulthood, and both of these effects were ameliorated through LSM supplementation. Differential regulation of various genes in the spinal cord of MS versus NS rats was observed, 41 of which were reversed by LSM supplementation. At the microbiota composition level MS led to changes in beta diversity and abundance of specific bacteria including parabacteroides, which were ameliorated by LSM. These findings support probiotic soluble mediators as potential interventions in the reduction of symptoms of visceral hypersensitivity.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Offspring
medicine.medical_treatment
Gut flora
law.invention
03 medical and health sciences
Probiotic
0302 clinical medicine
Lactobacillus rhamnosus
Microbiome-Gut-Brain axis
law
Lactobacillus
Internal medicine
Host-Microbe Interactions
medicine
Research Articles
biology
business.industry
Prebiotic
Probiotics
Visceral pain
Early life stress
biology.organism_classification
Gastrointestinal, Renal & Hepatic Systems
Pathophysiology
030104 developmental biology
Endocrinology
medicine.symptom
business
030217 neurology & neurosurgery
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 20596553
- Volume :
- 4
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Neuronal Signaling
- Accession number :
- edsair.doi.dedup.....aff7f0c62c8a40332b0265d4074df5c9