Back to Search
Start Over
Oral administration of Hsp65-producing Lactococcus lactis attenuates allergic asthma in a murine model
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2020
-
Abstract
- Aims Allergic asthma is a chronic inflammatory lung disease characterized by a Th2-type immune response pattern. The development of nonspecific immunotherapy is one of the primary goals for the control of this disease. Methods and results In this study, we evaluated the therapeutic effects of Lactococcus lactis-producing mycobacterial heat shock protein 65 (LLHsp65) in an ovalbumin (OVA)-induced allergic asthma model. OVA-challenged BALB/c mice were orally administrated with LLHsp65 for 10 consecutive days. The results demonstrate that LLhsp65 attenuates critical features of allergic inflammation, like airway hyperresponsiveness and mucus production. Likewise, the treatment decreases the pulmonary eosinophilia and the serum level of OVA-specific IgE. In addition to deviating immune responses towards Th1-cytokine profile, increase regulatory T cells, and cytokine levels, such as IL-6 and IL-10. Conclusions Our results reveal that the mucosal immunotherapy of LLHsp65 significantly reduces the overall burden of airway allergic inflammation, suggesting a promising therapeutic strategy for allergic asthma treatment. Significance and impact of the study This research reveals new perspectives on nonspecific immunotherapy based on the delivery of recombinant proteins by lactic acid bacteria to treat of allergic disorders.
- Subjects :
- Ovalbumin
medicine.medical_treatment
Administration, Oral
Immunoglobulin E
Applied Microbiology and Biotechnology
T-Lymphocytes, Regulatory
Allergic inflammation
Mice
Immune system
Bacterial Proteins
Heat shock protein
medicine
Hypersensitivity
Animals
Pulmonary Eosinophilia
Lung
HIPERSENSIBILIDADE
Inflammation
Mice, Inbred BALB C
biology
business.industry
General Medicine
Immunotherapy
Chaperonin 60
respiratory system
Asthma
Lactococcus lactis
Disease Models, Animal
Cytokine
Immunoglobulin G
Immunology
biology.protein
Cytokines
Female
business
Bronchoalveolar Lavage Fluid
Biotechnology
Subjects
Details
- ISSN :
- 13652672
- Volume :
- 130
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of applied microbiologyReferences
- Accession number :
- edsair.doi.dedup.....afefef4fb6eab0c4f01a37d1a4745a78