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Mesenchymal cells from human amniotic fluid survive and migrate after transplantation into adult rat brain
- Source :
- Cell Biology International. 31:845-850
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- Amniotic fluid has been recently suggested as an alternative source of mesenchymal stem cells. However, the fate of amniotic fluid-derived mesenchymal stem cells (AF-MSCs) after in vivo transplantation has yet to be determined. In the present study we explored whether human AF-MSCs could survive and migrate following transplantation into the striatum of normal and ischemic rat. We found that the grafted cells could survive and migrate towards multiple brain regions in the normal animals, while they moved towards the injured region in the ischemic rat. Double-immunostaining analyses showed that the implanted human AF-MSCs express markers for immature neurons (Doublecortin) at 10 days, and for astrocytes (GFAP) at 10, 30 and 90 after transplantation. This study provides the first evidence that human amniotic fluid contains cells having the potential to survive and integrate into adult rat brain tissue and, therefore, to function as effective stem cells for therapeutic strategies.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Doublecortin Protein
Amniotic fluid
Cell Survival
Transplantation, Heterologous
Clinical uses of mesenchymal stem cells
Biology
Mesenchymal Stem Cell Transplantation
Brain Ischemia
Rats, Sprague-Dawley
Cell Movement
medicine
Animals
Humans
Cells, Cultured
Stem cell transplantation for articular cartilage repair
Microscopy, Confocal
Mesenchymal stem cell
Mesenchymal Stem Cells
Amniotic stem cells
Cell Biology
General Medicine
Amniotic Fluid
Rats
Transplantation
Amniotic epithelial cells
Immunology
Stem cell
Subjects
Details
- ISSN :
- 10656995
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Cell Biology International
- Accession number :
- edsair.doi.dedup.....afc49aa6a19b475dae519f065537cbad