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Recombinant xylanase from Streptomyces coelicolor Ac-738: characterization and the effect on xylan-containing products
- Source :
- World Journal of Microbiology and Biotechnology. 30:801-808
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- A xylanase gene was isolated from the genomic DNA of Streptomyces coelicolor Ac-738. The 723-bp full-length gene encoded a 241-amino acid peptide consisting of a 49-residue putative TAT signal peptide and a glycoside hydrolase family-11 domain. The mature enzyme called XSC738 was expressed in Escherichia coli M15[pREP4]. The electrophoretically homogeneous protein with a specific activity of 167 U/mg for beechwood xylan was purified. The pH optimum of XSC738 was at pH 6; a high activity was retained within a pH range of 4.5-8.5. The enzyme was thermostable at 50-60 °C and retained an activity at pH 3.0-7.0. Xylanase XSC738 was activated by Mn²⁺, Co²⁺ and largely inhibited by Cd²⁺, SDS and EDTA. The products of xylan hydrolysis were mainly xylobiose, xylotriose, xylopentaose and xylohexose. Xylotetraose appeared as a minor product. Processing of such agricultural xylan-containing products as wheat, oats, soy flour and wheat bran by xylanase resulted in an increased content of sugars.
- Subjects :
- Avena
Physiology
Enzyme Activators
Gene Expression
Streptomyces coelicolor
Applied Microbiology and Biotechnology
chemistry.chemical_compound
Hydrolysis
Enzymatic hydrolysis
Enzyme Stability
Escherichia coli
Xylobiose
Glycoside hydrolase
Enzyme Inhibitors
Triticum
chemistry.chemical_classification
biology
Temperature
food and beverages
General Medicine
Hydrogen-Ion Concentration
biology.organism_classification
Xylan
Recombinant Proteins
Molecular Weight
Xylosidases
Enzyme
Biochemistry
chemistry
Metals
Xylanase
Xylans
Biotechnology
Subjects
Details
- ISSN :
- 15730972 and 09593993
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- World Journal of Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....afc471407cf347afee1ea4d54b3d2441