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Integrator complex subunit 6 (INTS6) inhibits hepatocellular carcinoma growth by Wnt pathway and serve as a prognostic marker
- Source :
- BMC Cancer, Vol 17, Iss 1, Pp 1-12 (2017), BMC Cancer
- Publication Year :
- 2017
- Publisher :
- BMC, 2017.
-
Abstract
- Background Integrator complex subunit 6 (INTS6) was found to play a tumour suppressing role in certain types of solid tumours. In this study, we wanted to determine the expression level of INTS6 in hepatocellular carcinoma (HCC) and evaluate its clinical characteristics and mechanisms in HCC patients (Lui and Lu, European Journal of Cancer, 51:S94, 2015). Methods First, we used a microarray analysis to explore the mRNA expression levels in HCC and paired normal liver tissues; second, we used qRT-PCR to measure the INTS6 mRNA levels in a cohort of 50 HCC tissues and adjacent normal liver tissues; third, we used Western blot analyses to detect the INTS6 protein levels in 20 paired HCC and normal liver tissues; fourth, we used immunohistochemistry to determine the INTS6 expression levels in 70 archived paraffin-embedded HCC samples. Finally, we investigated the suppressive function of INTS6 in the Wnt pathway. Results Herein, according to the microarray data analysis, the expression levels of INTS6 were dramatically down-regulated in HCC tissues vs. those in normal liver tissues (p
- Subjects :
- Male
0301 basic medicine
Cancer Research
Pathology
Hepatocellular carcinoma
Kaplan-Meier Estimate
0302 clinical medicine
Surgical oncology
Wnt Signaling Pathway
Wnt/β-catenin
medicine.diagnostic_test
Liver Neoplasms
Wnt signaling pathway
RNA-Binding Proteins
Middle Aged
Prognosis
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Gene Expression Regulation, Neoplastic
Oncology
030220 oncology & carcinogenesis
Immunohistochemistry
Female
Research Article
Adult
Ribosomal Proteins
medicine.medical_specialty
Carcinoma, Hepatocellular
lcsh:RC254-282
Disease-Free Survival
03 medical and health sciences
Western blot
Biomarkers, Tumor
Genetics
medicine
Humans
Adaptor Proteins, Signal Transducing
Aged
Cell Proliferation
Messenger RNA
Microarray analysis techniques
business.industry
Tumor Suppressor Proteins
Cancer
medicine.disease
Repressor Proteins
030104 developmental biology
Cancer research
business
INTS6
Subjects
Details
- Language :
- English
- ISSN :
- 14712407
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....afc261a50898c08a5eb483e8173e4a4e