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MIFlowCyt‐EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments
- Source :
- Journal of Extracellular Vesicles 1 (9), . (2020), Journal of Extracellular Vesicles, Journal of Extracellular Vesicles, Taylor & Francis, 2020, 9 (1), ⟨10.1080/20013078.2020.1713526⟩, Journal of Extracellular Vesicles, 9(1). Co-Action Publishing, Journal of Extracellular Vesicles, Vol 9, Iss 1 (2020), Journal of Extracellular Vesicles, 2020, 9 (1), ⟨10.1080/20013078.2020.1713526⟩, JOURNAL OF EXTRACELLULAR VESICLES, Journal of extracellular vesicles, 9(1):1713526. Taylor and Francis Ltd.
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- International audience; Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and has produced a number of controversial results, in part due to limitations of instrument detection, lack of robust methods and ambiguities in how data should be interpreted. These complications are exacerbated by the field's lack of a robust reporting framework, and many EV-FC manuscripts include incomplete descriptions of methods and results, contain artefacts stemming from an insufficient instrument sensitivity and inappropriate experimental design and lack appropriate calibration and standardization. To address these issues, a working group (WG) of EV-FC researchers from ISEV, ISAC and ISTH, worked together as an EV-FC WG and developed a consensus framework for the minimum information that should be provided regarding EV-FC. This framework incorporates the existing Minimum Information for Studies of EVs (MISEV) guidelines and Minimum Information about a FC experiment (MIFlowCyt) standard in an EV-FC-specific reporting framework (MIFlowCyt-EV) that supports reporting of critical information related to sample staining, EV detection and measurement and experimental design in manuscripts that report EV-FC data. MIFlowCyt-EV provides a structure for sharing EV-FC results, but it does not prescribe specific protocols, as there will continue to be rapid evolution of instruments and methods for the foreseeable future. MIFlowCyt-EV accommodates this evolution, while providing information needed to evaluate and compare different approaches. Because MIFlowCyt-EV will ensure consistency in the manner of reporting of EV-FC studies, over time we expect that adoption of MIFlowCyt-EV as a standard for reporting EV- FC studies will improve the ability to quantitatively compare results from different laboratories and to support the development of new instruments and assays for improved measurement of EVs.
- Subjects :
- 0301 basic medicine
Histology
Standardization
Computer science
SOCIETY
Medizin
Médecine humaine et pathologie
cytométrie de flux
standardisation
Sample (statistics)
Extracellular vesicles
Field (computer science)
03 medical and health sciences
Consistency (database systems)
0302 clinical medicine
framework
Medicine and Health Sciences
lcsh:QH573-671
standardization
reporting
Measure (data warehouse)
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
lcsh:Cytology
MICROPARTICLES
flow cytometry
vésicule extracellulaire
Cell Biology
Extracellular vesicle
Data science
030104 developmental biology
030220 oncology & carcinogenesis
CELLS
Key (cryptography)
Human health and pathology
Position Paper
SENSITIVITY
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Subjects
Details
- ISSN :
- 20013078
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of Extracellular Vesicles
- Accession number :
- edsair.doi.dedup.....afc14786f28686f9884419a3d7ae012e