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MIFlowCyt‐EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

Authors :
Welsh, Joshua A
Van Der Pol, Edwin
Arkesteijn, Ger J A
Bremer, Michel
Brisson, Alain
Coumans, Frank
Dignat-George, Françoise
Duggan, Erika
Ghiran, Ionita
Giebel, Bernd
Görgens, André
Hendrix, An
Lacroix, Romaric
Lannigan, Joanne
Libregts, Sten F W M
Lozano-Andrés, Estefanía
Morales-Kastresana, Aizea
Robert, Stephane
De Rond, Leonie
Tertel, Tobias
Tigges, John
De Wever, Olivier
Yan, Xiaomei
Nieuwland, Rienk
Wauben, Marca H M
Nolan, John P
Jones, Jennifer C
LS Celbiologie-Algemeen
Celbiologie
dB&C I&I
ProdInra, Migration
National Cancer Institute
Vrije Universiteit Amsterdam [Amsterdam] (VU)
Utrecht University [Utrecht]
University of Duisberg Essen
Partenaires INRAE
Université Bordeaux
Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN)
Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Hôpital de la Conception [CHU - APHM] (LA CONCEPTION)
The Scintillon Institute
Harvard Medical School [Boston] (HMS)
Evox Therapeutics Limited
University of Duisburg-Essen
Karolinska Institutet [Stockholm]
Ghent University Hospital
Aix Marseille Université (AMU)
University of Virginia
University of Cambridge [UK] (CAM)
Beth Israel Deaconess Medical Center
Xiamen University
1ZIA-BC011502
NIH UG3 TR002881
VENI 13681 VENI 15924
NIH UG3 TR002881 U01-126497 U01-OD -019750 R01 CA218500 R01 HL1266497
LS Celbiologie-Algemeen
Celbiologie
dB&C I&I
VU University Amsterdam
Vrije universiteit = Free university of Amsterdam [Amsterdam] (VU)
Universität Duisburg-Essen = University of Duisburg-Essen [Essen]
Biomedical Engineering and Physics
ACS - Atherosclerosis & ischemic syndromes
CCA - Imaging and biomarkers
ACS - Microcirculation
Graduate School
Laboratory Specialized Diagnostics & Research
Source :
Journal of Extracellular Vesicles 1 (9), . (2020), Journal of Extracellular Vesicles, Journal of Extracellular Vesicles, Taylor & Francis, 2020, 9 (1), ⟨10.1080/20013078.2020.1713526⟩, Journal of Extracellular Vesicles, 9(1). Co-Action Publishing, Journal of Extracellular Vesicles, Vol 9, Iss 1 (2020), Journal of Extracellular Vesicles, 2020, 9 (1), ⟨10.1080/20013078.2020.1713526⟩, JOURNAL OF EXTRACELLULAR VESICLES, Journal of extracellular vesicles, 9(1):1713526. Taylor and Francis Ltd.
Publication Year :
2020
Publisher :
Wiley, 2020.

Abstract

International audience; Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and has produced a number of controversial results, in part due to limitations of instrument detection, lack of robust methods and ambiguities in how data should be interpreted. These complications are exacerbated by the field's lack of a robust reporting framework, and many EV-FC manuscripts include incomplete descriptions of methods and results, contain artefacts stemming from an insufficient instrument sensitivity and inappropriate experimental design and lack appropriate calibration and standardization. To address these issues, a working group (WG) of EV-FC researchers from ISEV, ISAC and ISTH, worked together as an EV-FC WG and developed a consensus framework for the minimum information that should be provided regarding EV-FC. This framework incorporates the existing Minimum Information for Studies of EVs (MISEV) guidelines and Minimum Information about a FC experiment (MIFlowCyt) standard in an EV-FC-specific reporting framework (MIFlowCyt-EV) that supports reporting of critical information related to sample staining, EV detection and measurement and experimental design in manuscripts that report EV-FC data. MIFlowCyt-EV provides a structure for sharing EV-FC results, but it does not prescribe specific protocols, as there will continue to be rapid evolution of instruments and methods for the foreseeable future. MIFlowCyt-EV accommodates this evolution, while providing information needed to evaluate and compare different approaches. Because MIFlowCyt-EV will ensure consistency in the manner of reporting of EV-FC studies, over time we expect that adoption of MIFlowCyt-EV as a standard for reporting EV- FC studies will improve the ability to quantitatively compare results from different laboratories and to support the development of new instruments and assays for improved measurement of EVs.

Details

ISSN :
20013078
Volume :
9
Database :
OpenAIRE
Journal :
Journal of Extracellular Vesicles
Accession number :
edsair.doi.dedup.....afc14786f28686f9884419a3d7ae012e