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Arabidopsis SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2 inhibit WRKY75 function in abscisic acid-mediated leaf senescence and seed germination
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- The plant-specific VQ gene family participates in diverse physiological processes but little information is available on their role in leaf senescence. Here, we show that the VQ motif-containing proteins, Arabidopsis SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2 are negative regulators of abscisic acid (ABA)-mediated leaf senescence. Loss of SIB1 and SIB2 function resulted in increased sensitivity of ABA-induced leaf senescence. In contrast, overexpression of SIB1 significantly delayed this process. Moreover, biochemical studies revealed that SIBs interact with WRKY75 transcription factor. Loss of WRKY75 function decreased sensitivity to ABA-induced leaf senescence, while overexpression of WRKY75 significantly accelerated this process. Chromatin immunoprecipitation assays revealed that WRKY75 directly binds to the promoters of GOLDEN 2-LIKE1(GLK1) and GLK2, to repress their expression. SIBs repress the transcriptional function of WRKY75 and negatively regulate ABA-induced leaf senescence in a WRKY75-dependent manner. In contrast, WRKY75 positively modulates ABA-mediated leaf senescence in a GLK-dependent manner. In addition, SIBs inhibit WRKY75 function in ABA-mediated seed germination. These results demonstrate that SIBs can form a complex with WRKY75 to regulate ABA-mediated leaf senescence and seed germination.<br />SIBs function as repressors of WRKY75 and regulate expression of GLKs in ABA-mediated leaf senescence and seed germination.
- Subjects :
- Senescence
WRKY75
leaf senescence
Physiology
Arabidopsis
seed germination
Germination
Sigma Factor
Plant Science
Biology
Abscisic acid
chemistry.chemical_compound
Gene Expression Regulation, Plant
SIGMA FACTOR BINDING PROTEIN
Sigma factor
Gene family
Transcription factor
Arabidopsis Proteins
AcademicSubjects/SCI01210
fungi
food and beverages
Promoter
biology.organism_classification
Research Papers
Plant Senescence
Cell biology
chemistry
Seeds
GOLDEN 2-LIKE1/2
Growth and Development
Chromatin immunoprecipitation
Protein Binding
Subjects
Details
- ISSN :
- 14602431 and 00220957
- Volume :
- 73
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....afc03d68188dd65828d91a182d28065b