Back to Search
Start Over
MicroRNA-101b attenuates cardiomyocyte hypertrophy by inhibiting protein kinase C epsilon signaling
- Source :
- FEBS letters. 591(1)
- Publication Year :
- 2016
-
Abstract
- Previously, a surgical regression model identified microRNA-101b (miR-101b) as a potential inhibitor of cardiac hypertrophy. Here, we investigated the antihypertrophic mechanism of miR-101b using neonatal rat ventricular myocytes. miR-101b markedly suppressed agonist-induced cardiac hypertrophy as shown by cell size and fetal gene expression. By systems biology approaches, we identified protein kinase C epsilon (PKCe) as the major target of miR-101b. Our results from qRT-PCR, western blot, and luciferase reporter assays confirm that PKCe is a direct target of miR-101b. In addition, we found that effectors downstream of PKCe (p-AKT, p-ERK1/2, p-NFAT, and p-GSK3β) are also affected by miR-101b. Our study reveals a novel inhibitory mechanism for miR-101b as a negative regulator of cardiac hypertrophy.
- Subjects :
- 0301 basic medicine
Biophysics
Protein Kinase C-epsilon
Cardiomegaly
Biology
Biochemistry
Rats, Sprague-Dawley
03 medical and health sciences
Western blot
Structural Biology
microRNA
Gene expression
Genetics
medicine
Animals
Myocytes, Cardiac
RNA, Messenger
Molecular Biology
Protein kinase C
medicine.diagnostic_test
Base Sequence
Endothelin-1
Effector
Cell Biology
Endothelin 1
Molecular biology
MicroRNAs
030104 developmental biology
Signal transduction
Signal Transduction
Subjects
Details
- ISSN :
- 18733468
- Volume :
- 591
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....afacbefc837e5e2df5ec75bf5c4fb040