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Progesterone modulates endothelium-dependent coronary vascular reactivity in SHR
- Source :
- Journal of molecular endocrinology. 66(2)
- Publication Year :
- 2020
-
Abstract
- Although progesterone has the ability to promote dilation of vascular smooth muscle, its role in coronary circulation is still poorly characterized, especially in essential hypertension and in a model of endogenous deficiency of ovarian hormones. Thus, this study evaluated the effect of progesterone treatment on endothelium-dependent coronary vascular reactivity in hypertensive (SHR) and ovariectomized rats. Adult SHR aged 8–10 weeks were divided into: SHAM, Ovariectomized (OVX) and Ovariectomized + treatment with 2 mg/kg/day of progesterone for 15 days (OVX-P4). Coronary vascular reactivity was investigated using the modified Langendorff method. After stabilization, baseline coronary perfusion pressure (CPP) was recorded and vascular reactivity to bradykinin (BK, 0.1–1000 ng) were assessed before and after infusion, either individually or in combination, with Nω-nitro-l-arginine methyl ester (l-NAME), indomethacin or clotrimazole. Scanning electron microscopy was used for qualitative analysis of the endothelium. OVX and OVX-P4 groups had a higher baseline CPP compared to that of the SHAM group. BK was able to promote vasodilation in all groups. However, relaxation to BK was less pronounced in the OVX group when compared to SHAM, with architecture loss and areas of cell atrophy having been observed. Progesterone treatment prevented this injury. Perfusion with l-NAME induced greater damage to the SHAM group, while the use of indomethacin led to a significant reduction in the vasodilator response to BK in the OVX-P4 group. Taken together, our results show that progesterone modulates endothelium-dependent coronary vasodilation in SHR ovariectomized, preventing damage caused by ovarian hormonal deficiency through a mechanism that involves prostanoid pathway.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Vascular smooth muscle
Endothelium
Systole
Vasodilator Agents
Bradykinin
030209 endocrinology & metabolism
Vasodilation
Blood Pressure
Essential hypertension
03 medical and health sciences
chemistry.chemical_compound
Coronary circulation
0302 clinical medicine
Endocrinology
Internal medicine
Rats, Inbred SHR
medicine
Animals
Molecular Biology
Progesterone
business.industry
Body Weight
Uterus
Organ Size
medicine.disease
Coronary Vessels
Perfusion
030104 developmental biology
medicine.anatomical_structure
chemistry
Hypertension
Ovariectomized rat
Coronary perfusion pressure
Female
Endothelium, Vascular
business
Subjects
Details
- ISSN :
- 14796813
- Volume :
- 66
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of molecular endocrinology
- Accession number :
- edsair.doi.dedup.....af9f34c863d8b1c7cd707e1c17b99f63