PCR analysis of the expression of chromosome 18 genes in human liver tissue: interindividual variability

Using human chromosome 18 (Ch18) genes as an example, a PCR analysis of the interindividual variability of gene expression in liver tissue was performed. Although the quantitative profiles of the Ch18 transcriptome, expressed in the number of cDNA copies per single cell, showed a high degree of correlation between donors (Pearson correlation coefficients ranged from 0.963 to 0.966), the expression of the significant number of genes (from 13% to 19%, depending on the method of experimental data normalization) varied by more than 4-fold when comparing donors pairwise. At the same time, the proportion of differentially expressed genes increased with a decrease in the level of their expression. It is shown that the higher quantitative variability of low-abundance transcripts is mainly not technical, but biological. Bioinformatic analysis of the interindividual variability of the differential expression of chromosome 18 genes in human liver tissue did not reveal any statistically significant groups of genes related to certain biological processes that indicated a rather transient nature of the interindividual variability of their expression, probably reflecting the response of cells of an individual to specific external stimuli.Na primere khromosomy 18 cheloveka proveden PTsR-analiz mezhindividual'noĭ variabel'nosti ékspressii 244 genov v tkani pecheni. V tselom kolichestvennye profili transkriptoma khromosomy 18, vyrazhennye v kolichestve kopiĭ kDNK na edinichnuiu kletku, pokazyvaiut vysokuiu stepen' korreliatsii (rp=0,963-0,966). Odnako pri poparnom sravnenii donorov ékspressiia sushchestvennogo kolichestva genov (ot 13% do 19% v zavisimosti ot sposoba normirovki éksperimental'nykh dannykh) razlichaetsia bolee chem 4-kratno. Pri étom dolia differentsial'no ékspressirovannykh genov vozrastaet s ponizheniem urovnia ikh ékspressii. Bolee vysokaia kolichestvennaia variabel'nost' nizkopredstavlennykh transkriptov imeet v osnovnom ne tekhnicheskuiu, a biologicheskuiu prichinu. Bioinformaticheskiĭ analiz mezhindividual'noĭ variabel'nosti differentsial'noĭ ékspressii genov khromosomy 18 v tkani pecheni cheloveka ne vyiavil kakikh-libo statisticheski znachimykh grupp genov, otnosiashchikhsia k opredelennym biologicheskim protsessam. Éto, po-vidimomu, ukazyvaet na vremennuiu prirodu mezhindividual'noĭ variabel'nosti ikh ékspressii, veroiatno, otrazhaiushchuiu reaktsiiu kletok dannogo individuuma na konkretnye vneshnie stimuly.