Back to Search Start Over

Stb6 mediates stomatal immunity, photosynthetic functionality, and the antioxidant system during the Zymoseptoria tritici-wheat interaction

Authors :
Fateme Ghiasi Noei
Mojtaba Imami
Fardad Didaran
Mohammad Amin Ghanbari
Elham Zamani
Amin Ebrahimi
Sasan Aliniaeifard
Mohsen Farzaneh
Mohammad Javan-Nikkhah
Angela Feechan
Amir Mirzadi Gohari
Source :
Frontiers in Plant Science. 13
Publication Year :
2022
Publisher :
Frontiers Media SA, 2022.

Abstract

This study offers new perspectives on the biochemical and physiological changes that occur in wheat following a gene-for-gene interaction with the fungal pathogen Zymoseptoria tritici. The Z. tritici isolate IPO323, carries AvrStb6, while ΔAvrStb6#33, lacks AvrStb6. The wheat cultivar (cv.) Shafir, bears the corresponding resistance gene Stb6. Inoculation of cv. Shafir with these isolates results in two contrasted phenotypes, offering a unique opportunity to study the immune response caused by the recognition of AvrStb6 by Stb6. We employed a variety of methodologies to dissect the physiological and biochemical events altered in cv. Shafir, as a result of the AvrStb6-Stb6 interaction. Comparative analysis of stomatal conductance demonstrated that AvrStb6-Stb6 mediates transient stomatal closures to restrict the penetration of Zymoseptoria tritici. Tracking photosynthetic functionality through chlorophyll fluorescence imaging analysis demonstrated that AvrStb6-Stb6 retains the functionality of photosynthesis apparatus by promoting Non-Photochemical Quenching (NPQ). Furthermore, the PlantCV image analysis tool was used to compare the H2O2 accumulation and incidence of cell death (2, 4, 8, 12, 16, and 21 dpi), over Z. tritici infection. Finally, our research shows that the AvrStb6-Stb6 interaction coordinates the expression and activity of antioxidant enzymes, both enzymatic and non-enzymatic, to counteract oxidative stress. In conclusion, the Stb6-AvrStb6 interaction in the Z. tritici-wheat pathosystem triggers transient stomatal closure and maintains photosynthesis while regulating oxidative stress.

Subjects

Subjects :
Plant Science

Details

ISSN :
1664462X
Volume :
13
Database :
OpenAIRE
Journal :
Frontiers in Plant Science
Accession number :
edsair.doi.dedup.....af9a065efcfbd627c8be1a6c80f0d5d5
Full Text :
https://doi.org/10.3389/fpls.2022.1004691