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Regulation of actin dynamics by annexin 2
- Source :
- The EMBO journal. 25(9)
- Publication Year :
- 2005
-
Abstract
- Annexin 2 is a ubiquitous Ca(2+)-binding protein that is essential for actin-dependent vesicle transport. Here, we show that in spontaneously motile cells annexin 2 is concentrated in dynamic actin-rich protrusions, and that depletion of annexin 2 using siRNA leads to the accumulation of stress fibres and loss of protrusive and retractile activity. Cells co-expressing annexin 2-CFP and actin-YFP exhibit Ca(2+)-dependent fluorescense resonance energy transfer throughout the cytoplasm and in membrane ruffles and protrusions, suggesting that annexin 2 may directly interact with actin. This notion was supported by biochemical studies, in which we show that annexin 2 reduces the polymerisation rate of actin monomers in a dose-dependent manner. By measuring actin polymerisation rates in the presence of barbed-end and pointed-end cappers, we further demonstrate that annexin 2 specifically inhibits filament elongation at the barbed ends. These results show that annexin 2 has an essential role in maintaining the plasticity of the dynamic membrane-associated actin cytoskeleton, and that its activity in this context may be at least partly explained through direct interactions with polymerised and monomeric actin.
- Subjects :
- Cytoplasm
Arp2/3 complex
macromolecular substances
General Biochemistry, Genetics and Molecular Biology
Article
Actin remodeling of neurons
Annexin
Fluorescence Resonance Energy Transfer
Animals
Humans
Actin-binding protein
RNA, Small Interfering
Cytoskeleton
Molecular Biology
Cell Shape
Actin
Annexin A2
Cells, Cultured
General Immunology and Microbiology
biology
General Neuroscience
Cell Membrane
Actin cytoskeleton
Actins
Cell biology
Actin Cytoskeleton
Luminescent Proteins
biology.protein
Rabbits
Subjects
Details
- ISSN :
- 02614189
- Volume :
- 25
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- The EMBO journal
- Accession number :
- edsair.doi.dedup.....af91a3c35b6a72f4790f95fd7a0788a9