Back to Search
Start Over
Effect of auranofin, a new antiarthritic agent, on immune complex-induced release of lysosomal enzymes from human leukocytes
- Source :
- Inflammation. 2(2)
- Publication Year :
- 1977
-
Abstract
- Auranofin, an oral chrysotherapeutic agent effective in the treatment of rheumatoid arthritis (RA), was found to be a potent, noncytotoxic inhibitor of IgG-RF immune complex-induced lysosomal enzyme release (LER) from human leukocytes. At a concentration of 1 microg Au/ml (5 microM), auranofin produced a marked reduction in beta-glucuronidase (100%), acid phosphatase (88%), and lysozyme (72%) release. In contrast, gold sodium thiosulfate (GST, an injectable gold compound) had no inhibitory activity on LER at equivalent gold concentrations (i.e., 1 microg Au/ml) and only modest activity (less than 36% inhibition) at concentrations as high as 40 microg Au/ml. The 50% inhibitory dose (LD50) of auranofin on LER was calculated to be 3-4 microM (0.6-0.8 microg Au/ml). Blood gold levels in auranofin-treated RA patients were found to be within the range required for in vitro inhibition of LER, and correlated with decreases in IgG, RF titers, and IgG-RF immune-complex formation in vitro. These results suggest that the therapeutic action of auranofin may be caused, at least in part, by inhibition of LER and/or decreases in immune-complex formation.
- Subjects :
- Auranofin
Immunology
Antigen-Antibody Complex
Pharmacology
In Vitro Techniques
Gold Sodium Thiomalate
chemistry.chemical_compound
Immune system
Phagocytosis
Rheumatoid Factor
medicine
Leukocytes
Organometallic Compounds
Immunology and Allergy
Humans
chemistry.chemical_classification
biology
Antiarthritic agent
Acid phosphatase
food and beverages
In vitro
Immune complex
Enzyme
chemistry
Biochemistry
Immunoglobulin G
biology.protein
Gold
Lysozyme
Lysosomes
medicine.drug
Subjects
Details
- ISSN :
- 03603997
- Volume :
- 2
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Inflammation
- Accession number :
- edsair.doi.dedup.....af88a24a876f88277e8a0a649f6ccbec