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Repeated-batch fermentation using flocculent hybrid, Saccharomyces cerevisiae CHFY0321 for efficient production of bioethanol
- Source :
- Applied Microbiology and Biotechnology. 84:261-269
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- In this study, the repeated-batch fermentation of liquefied cassava medium using the flocculent hybrid Saccharomyces cerevisiae CHFY0321 was investigated for semicontinuous, high-throughput production of bioethanol. Cassava medium was selected due to the industrial requirement for a cheap starchy substrate. Fermentations were performed by simultaneous saccharification and fermentation (SSF) with a set of ten batches successfully completing a series within the repeated fermentation process. In addition, pH of the culture medium was not controlled to simplify ethanol production for future use in industry. Optimal recycling volume was found to be 5%. Volumetric productivity, final ethanol concentration, and ethanol yield were measured at 3.34 g l(-1) h(-1), 84.5 g l(-1), and 90.7%, respectively. Cell recycling (24.5 g DCW l(-1)) resulted in 1.8-fold decrease in fermentation time (24 h) and 1.8-fold increase in volumetric productivity compared with the ordinary batch fermentation. Therefore, repeated-batch SSF using flocculent CHFY0321 demonstrates the possibility of cost-effective bioethanol production by eliminating additional saccharification and inoculation steps.
- Subjects :
- Manihot
Batch fermentation
Protein Hydrolysates
Saccharomyces cerevisiae
Carbohydrates
Applied Microbiology and Biotechnology
Hydrolysis
chemistry.chemical_compound
Ethanol fuel
Ethanol
biology
Chemistry
business.industry
Flocculation
food and beverages
General Medicine
biology.organism_classification
Pulp and paper industry
Yeast
Culture Media
Biotechnology
Biofuel
Fermentation
business
Subjects
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....af7ecd0af434bcc95c756cac8cd42b9a