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DNA-loop-extruding SMC complexes can traverse one another in vivo
- Source :
- Nat Struct Mol Biol
- Publication Year :
- 2020
-
Abstract
- Chromosome organization mediated by structural maintenance of chromosomes (SMC) complexes is vital in many organisms. SMC complexes act as motors that extrude DNA loops, but it remains unclear what happens when multiple complexes encounter one another on the same DNA in living cells and how these interactions may help to organize an active genome. We therefore created a crash-course track system to study SMC complex encounters in vivo by engineering defined SMC loading sites in the Bacillus subtilis chromosome. Chromosome conformation capture (Hi-C) analyses of over 20 engineered strains show an amazing variety of chromosome folding patterns. Through three-dimensional polymer simulations and theory, we determine that these patterns require SMC complexes to bypass each other in vivo, as recently seen in an in vitro study. We posit that the bypassing activity enables SMC complexes to avoid traffic jams while spatially organizing the genome.
- Subjects :
- DNA, Bacterial
Chromosomal Proteins, Non-Histone
Cell Cycle Proteins
Bacillus subtilis
Genome
Article
Chromosome conformation capture
chemistry.chemical_compound
Structural Biology
In vivo
Molecular Biology
biology
Chromosome
Chromosome Organization
biology.organism_classification
musculoskeletal system
Cell biology
Folding (chemistry)
DNA-Binding Proteins
chemistry
Multiprotein Complexes
cardiovascular system
Nucleic Acid Conformation
DNA
Genome, Bacterial
Subjects
Details
- ISSN :
- 15459985
- Volume :
- 28
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Nature structuralmolecular biology
- Accession number :
- edsair.doi.dedup.....af7a5daee150c73d134b22eb1763f31e