Back to Search
Start Over
Effect of toluene exposure on the antioxidant status and apoptotic pathway in organs of the rat
- Source :
- British journal of biomedical science. 65(2)
- Publication Year :
- 2008
-
Abstract
- The chronic abuse of the solvent toluene results in structural and functional impairment of various organs. However, the pathophysiological mechanisms that cause these impairments of function are not clearly understood. This study aims to assess the effect of chronic toluene exposure (15, 30 and 45 days) on the oxidative stress and antioxidant status of different organs in the rat. Also, cyclooxygenase-2 and caspase-3 activities (a marker of apoptosis) are studied. Forty male albino rats were used and divided into four groups: controls (group I) and three other groups receiving a single daily dose of toluene (650 mg/kg) for 15 days (group II), 30 days (group III) and 45 days (group IV). The animals were then sacrificed and the brain cortex, cerebellum, liver, kidney and testis were separated for the determination of thiobarbituric acid reactive substance (TBARS), GSH, glutathione disulphide (GSSG) and glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD), cyclooxygenase-2 (COX-2) and caspase-3 activity. Results showed a significant and time-dependent increase in the levels of TBARS, GSSG and in GST, SOD, COX-2 and caspase-3 activity, while GSH, GR and GPx showed a marked decline in most tissues. The brain (cortex and cerebellum) was the most affected organ, showing the greatest increase in one apoptotic marker (caspase-3), while the testis and kidneys were least affected. In conclusion, oxidative stress and derangement of the GSH:GSSG ratio, induced chronic inflammatory change and apoptosis may play an essential role in toluene toxicity
- Subjects :
- Male
Antioxidant
medicine.medical_treatment
Clinical Biochemistry
Glutathione reductase
Apoptosis
medicine.disease_cause
Kidney
Antioxidants
chemistry.chemical_compound
Random Allocation
Testis
Immunology and Allergy
Toxicity Tests, Chronic
chemistry.chemical_classification
Toluene toxicity
biology
Caspase 3
Glutathione peroxidase
Brain
Infectious Diseases
Glutathione Reductase
Biochemistry
Liver
Microbiology (medical)
medicine.medical_specialty
Immunology
Microbiology
Superoxide dismutase
Internal medicine
medicine
TBARS
Animals
Rats, Wistar
Brain Chemistry
Analysis of Variance
Glutathione Peroxidase
Superoxide Dismutase
Biochemistry (medical)
Glutathione
Rats
Oxidative Stress
Endocrinology
chemistry
Prostaglandin-Endoperoxide Synthases
biology.protein
Solvents
Oxidative stress
Biomarkers
Toluene
Subjects
Details
- ISSN :
- 09674845
- Volume :
- 65
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- British journal of biomedical science
- Accession number :
- edsair.doi.dedup.....af3a929a67bbfaab2c4033a3120a3a74