Study of the nature of the binding sites of some allergens interacting with IgE

The nature of one of the binding sites of interacting macromolecules was studied by employing a model system consisting of immobilized allergens and specific reaginic antibodies present in the sera of allergen sensitive patients. Prior to their interaction with reaginic antibodies, the immobilized allergens were treated with a variety of enzymes in order to determine their susceptibility to enzymatic hydrolysis.