Back to Search
Start Over
Analysis of a newly discovered antigen of Bacillus cereus biovar anthracis for its suitability in specific serological antibody testing
- Source :
- Journal of applied microbiology. 126(1)
- Publication Year :
- 2018
-
Abstract
- Aims The aim of this work was to identify a protein which can be used for specific detection of antibodies against Bacillus cereus biovar anthracis (Bcbva), an anthrax-causing pathogen that so far has been described in African rainforest areas. Methods and results Culture supernatants of Bcbva and classic Bacillus anthracis (Ba) were analysed by gel electrophoresis, and a 35-kDa protein secreted only by Bcbva and not Ba was detected. The protein was identified as pXO2-60 by mass spectrometry. Sequence analysis showed that Ba is unable to secrete this protein due to a premature stop codon in the sequence for the signal peptide. Immunization of five outbred mice with sterile bacterial culture supernatants of Bcbva revealed an immune response in ELISA against pXO2-60 (three mice positive, one borderline) and the protective antigen (PA; four mice). When supernatants of classic Ba were injected into mice or human sera from anthrax patients were analysed, only antibodies against PA were detected. Conclusions In combination with PA, the pXO2-60 protein can be used for the detection of antibodies specific against Bcbva and discriminating from Ba. Significance and impact of the study After further validation, serological assays based on pXO2-60 can be used to perform seroprevalence studies to determine the epidemiology of B. cereus bv anthracis in affected countries and assess its impact on the human population.
- Subjects :
- Biovar
Population
Bacillus cereus
Applied Microbiology and Biotechnology
Microbiology
Serology
Anthrax
03 medical and health sciences
Mice
Blood serum
Antigen
Species Specificity
Animals
Humans
Serologic Tests
education
030304 developmental biology
0303 health sciences
education.field_of_study
Antigens, Bacterial
biology
030306 microbiology
fungi
General Medicine
biology.organism_classification
Antibodies, Bacterial
Bacillus anthracis
biology.protein
Antibody
Biotechnology
Subjects
Details
- ISSN :
- 13652672
- Volume :
- 126
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of applied microbiology
- Accession number :
- edsair.doi.dedup.....af2bcb2dff8f39cb65e077f88c29b422