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Synthesis of a Pyridyl Disulfide End-Functionalized Glycopolymer for Conjugation to Biomolecules and Patterning on Gold Surfaces
- Publication Year :
- 2009
-
Abstract
- A pyridyl disulfide end-functionalized polymer with N-acetyl-d-glucosamine pendant side-chains was synthesized by atom transfer radical polymerization (ATRP). The glycopolymer was prepared from a pyridyl disulfide initiator catalyzed by a Cu(I)/Cu(II)/2,2'-bipyridine system in a mixture of methanol and water at 30 degrees C. The final polymer had a number-average molecular weight (M(n)) of 13.0 kDa determined by (1)H NMR spectroscopy and a narrow polydispersity index (1.12) determined by gel permeation chromatography (GPC). The pyridyl disulfide end-group was then utilized to conjugate the glycopolymer to a double-stranded short interfering RNA (siRNA). Characterization of the glycopolymer-siRNA by polyacrylamide gel electrophoresis (PAGE) showed 97% conjugation. The activated disulfide polymer was also patterned on gold via microcontact printing. The pyridyl disulfide allowed for ready immobilization of the glycopolymer into 200 microm sized features on the surface.
- Subjects :
- Magnetic Resonance Spectroscopy
Polymers and Plastics
Polymers
Surface Properties
Glycopolymer
Dispersity
Bioengineering
Biocompatible Materials
Article
Biomaterials
Gel permeation chromatography
chemistry.chemical_compound
2,2'-Dipyridyl
Polysaccharides
Polymer chemistry
Materials Chemistry
Disulfides
RNA, Small Interfering
chemistry.chemical_classification
Atom-transfer radical-polymerization
Polymer
End-group
chemistry
Microcontact printing
Chromatography, Gel
Gold
Conjugate
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....af1d2fab07369e0a5d04c8ee686d8b9d