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Classification of Hepatitis C Virus Type 2 Isolates by Phylogenetic Analysis of Core and NS5 Regions

Classification of Hepatitis C Virus Type 2 Isolates by Phylogenetic Analysis of Core and NS5 Regions

Authors :
Claudia Fornai
A Morrica
Maria Linda Vatteroni
Mauro Bendinelli
Alessandro Leonildi
Mauro Pistello
Fabrizio Maggi
Source :
Journal of Clinical Microbiology. 37:2116-2117
Publication Year :
1999
Publisher :
American Society for Microbiology, 1999.

Abstract

Two methods for genotyping hepatitis C virus (DNA enzyme immunoassay [DEIA] and line probe assay [Inno-LiPA HCV I and II]) were compared on 120 samples and of these 87% were assigned to the same subtype by both assays. There were 15 subtyping discrepancies which involved 5% of type 1 isolates and 90% of type 2 isolates. Amplified products from the core and 5′ untranslated regions (UTR) were sequenced to resolve conflicts. Type 1 discordant samples had a guanosine at position −99 in the 5′ UTR, a characteristic of genotype 1b, and a core region typical of subtype 1a. The eight isolates classified as 2a/2c by LiPA and as subtype 2c by DEIA belonged to type 2.

Details

ISSN :
1098660X and 00951137
Volume :
37
Database :
OpenAIRE
Journal :
Journal of Clinical Microbiology
Accession number :
edsair.doi.dedup.....af127c2dcedf94d5575e8d7fa66fe7ee
Full Text :
https://doi.org/10.1128/jcm.37.6.2116-2117.1999