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Classification of Hepatitis C Virus Type 2 Isolates by Phylogenetic Analysis of Core and NS5 Regions
Classification of Hepatitis C Virus Type 2 Isolates by Phylogenetic Analysis of Core and NS5 Regions
- Source :
- Journal of Clinical Microbiology. 37:2116-2117
- Publication Year :
- 1999
- Publisher :
- American Society for Microbiology, 1999.
-
Abstract
- Two methods for genotyping hepatitis C virus (DNA enzyme immunoassay [DEIA] and line probe assay [Inno-LiPA HCV I and II]) were compared on 120 samples and of these 87% were assigned to the same subtype by both assays. There were 15 subtyping discrepancies which involved 5% of type 1 isolates and 90% of type 2 isolates. Amplified products from the core and 5′ untranslated regions (UTR) were sequenced to resolve conflicts. Type 1 discordant samples had a guanosine at position −99 in the 5′ UTR, a characteristic of genotype 1b, and a core region typical of subtype 1a. The eight isolates classified as 2a/2c by LiPA and as subtype 2c by DEIA belonged to type 2.
- Subjects :
- Microbiology (medical)
Hepatitis C Antigens
Hepatitis C Virus
Viral Core Proteins
Phylogenetic tree
biology
Hepatitis C virus
Hepacivirus
Viral Nonstructural Proteins
medicine.disease_cause
biology.organism_classification
Virology
Type (biology)
Italy
Phylogenetics
HCV
medicine
Humans
Phylogenetic relationships
Phylogeny
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....af127c2dcedf94d5575e8d7fa66fe7ee
- Full Text :
- https://doi.org/10.1128/jcm.37.6.2116-2117.1999